CORE--PROTEOMICS

核心--蛋白质组学

• 批准号: 7310757
• 负责人: CHRISTOPH H BORCHERS
• 金额: $ 13.79万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7310757
• 关键词: biomedical facility; molecular site; molecular weight; neoplasm /cancer; phosphorylation; posttranslational modifications; protein quantitation /detection; protein structure; protein structure function; proteomics; structural biology; ubiquitin

The goal of the Proteomics Core is to provide mass spectrometry-based protein identification and protein characterization services to UNC researchers. These services include protein identification from gels, and solution, identification of co-immunoprecipitated proteins, and protein characterization, including mapping of phosphorylation sites. Recently, in response to the need of the researchers, the Core has implemented a new program in differential protein expression analysis, based on Amersham's DIGE technique. The Core is led by the faculty director, Dr. Christoph Borchers, the Core Director, Carol Parker, and the Assistant Director, Dr. Maria Warren. The Core adds value to the Center by supporting over twenty-one grant proposals for UNC LCCC members. Highlights of research supported by the Core include two 2003 Nature publications based on the work done in the Core: one Nature paper involved the identification of new member of the cullin complex which is involved in cell cycle regulation (for Dr. Y. Xiong); the other Nature publication involved the identification of a phosphorylation site which is critical for cellular mobility, which has implications for metastasis (for Dr. K. Jacobson). Future plans for the Core include expanding the differential protein expression program, adding to the current program in accurate molecular weight determination of intact proteins, and continuing method development for the determination of phosphorylation and ubiquitination sites.

蛋白质组学核心的目标是为北卡罗来纳大学研究人员提供基于质谱的蛋白质鉴定和蛋白质表征服务。这些服务包括凝胶和溶液中的蛋白质鉴定、免疫共沉淀蛋白质的鉴定以及蛋白质表征，包括磷酸化位点的绘图。最近，为了满足研究人员的需求，Core 在 Amersham 的 DIGE 技术的基础上实施了差异蛋白表达分析的新程序。核心由教务主任 Christoph Borchers 博士、核心主任 Carol Parker 和助理主任 Maria Warren 博士领导。核心通过支持超过二十一项拨款提案为中心增加了价值 北卡罗来纳大学 LCCC 成员。核心支持的研究亮点包括两篇基于核心所做工作的 2003 年《自然》出版物：一篇《自然》论文涉及识别参与细胞周期调节的 cullin 复合体的新成员（为 Y. Xiong 博士）；另一篇《自然》出版物涉及磷酸化位点的鉴定，该位点对细胞移动性至关重要，这对转移具有影响（K. Jacobson 博士）。核心的未来计划包括扩展差异蛋白表达程序，在当前程序中添加完整蛋白质的精确分子量测定，以及继续方法 确定磷酸化和泛素化位点的开发。