Molecular Biology and Cytometry Research Shared Resource

分子生物学和细胞计数研究共享资源

基本信息

批准号：
10177887
负责人：
Jones Kenneth
金额：
$ 14.36万
依托单位：
UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-05-01 至 2023-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10177887
关键词：
Area Biological Assay Biomedical Research Cancer Center Cancer Center Support Grant Cell Cycle Cells Complex Consultations Core Facility Cytometry DNA sequencing Data Data Collection Disease Doctor of Philosophy Electrophoresis Experimental Designs Fees and Charges Floor Flow Cytometry Gel Genes Genomics Goals Image Infrastructure Laboratories Location Malignant Neoplasms Mass Spectrum Analysis Measurement Metabolic Microscopy Molecular Biology Molecular Weight Nucleic Acids Nucleic acid sequencing Peer Review Peptides Post-Translational Protein Processing Preparation Proteins Proteomics Protocols documentation Research Research Personnel Resolution Resource Sharing Sampling Science Services Signal Transduction Site Technology Training base cancer cell functional genomics high throughput screening high throughput technology incentive program instrumentation liquid chromatography mass spectrometry member multimodality nano next generation protein expression screening searchable database service member

项目摘要

MOLECULAR BIOLOGY AND CYTOMETRY RESEARCH SHARED RESOURCE – ABSTRACT The Molecular Biology and Cytometry Research Shared Resource (MBCR SR) is an institutionally managed central core facility that assists investigators in all aspects of research, including initial project consultation, assistance with experimental design, training in protocols and instrumentation usage, sample preparation, data collection and assistance with analysis and interpretation of results. Under the direction of James Tomasek, PhD, the OUHSC Vice President for Research, the MBCR SR provides four important Core services to SCC members and other investigators: 1) the DNA Sequencing and Genomics Core provides investigators with access to high-fidelity, next-generation nucleic acid sequencing data; 2) the Mass Spectrometry and Proteomics Core provides proteomics support for biomolecule identification, including complex proteomics analysis, post-translational modification identification, high-resolution molecular weight determination, large biomolecular mass measurements, LC-UV-MS analysis (qualitative or quantitative), protein identification by proteolytic digest (in-gel or in solution), nano LC/MS/MS and database search, and peptide sequencing; 3) the Flow Cytometry and Imaging Core provides flow cytometry and microscopy support for subcellular studies on protein expression, cell cycle and cell signaling; and 4) the Cancer Functional Genomics Core provides cutting- edge technology and services for high-throughput live cell functional characterization of cancer cells, including reverse proteomics array establishment, absolute gene quantification and copy number determination, nucleic acid and protein electrophoresis and imaging, multimodal screening for high-throughput assay on plates, high- throughput, high-content screening of subcellular localized proteins in cells, metabolic analysis of live cells and quantification of clonogenic assay colonies of cancer cells. The MBCR SR occupies a total of 6,409 sf. of laboratory space on the 1st, 3rd and 4th floors in the Stanton L. Young Biomedical Research Center (BRC) facility on the OUHSC campus. This central location provides the 27 BRC-based SCC members with immediate access to this important infrastructure. An additional five SCC members are located in the adjacent Biomedical Sciences Building (BMSB), which has a dedicated internal walkway to BRC. In 2016, 22% of the total number of MBCR SR users were SCC members with peer-reviewed support. The facility also served 12 SCC members without peer-reviewed support. In 2016, SCC members constituted 32% of the total users of this centralized institutional facility, which is by far the largest usage by a department or disease-site group at OUHSC, highlighting the high utilization and impact that the facility has on advancing research at the SCC. An incentive program initiated by the SCC covers 20% of all charged fees for SCC members for services provided by all areas of the MBCR.

分子生物学和细胞计数研究共享资源 - 摘要分子生物学和细胞计数研究共享资源 (MBCR SR) 是一个由机构管理的中央核心设施，协助研究人员进行研究的各个方面，包括初始项目咨询，协助实验设计、方案和仪器使用培训、样品制备、数据在 James Tomasek 的指导下收集并协助分析和解释结果。博士，OUHSC 研究副总裁，MBCR SR 为 SCC 提供四项重要的核心服务成员和其他研究人员：1) DNA 测序和基因组学核心为研究人员提供获取高保真下一代核酸测序数据；2) 质谱分析和蛋白质组学核心为生物分子鉴定提供蛋白质组学支持，包括复杂的蛋白质组学分析、翻译后修饰鉴定、高分辨率分子量测定、大生物分子质量测量、LC-UV-MS 分析（定性或定量）、蛋白质鉴定蛋白水解消化（凝胶内或溶液中）、纳米 LC/MS/MS 和数据库搜索以及肽测序 3) 流式细胞术和成像核心为亚细胞研究提供流式细胞术和显微镜支持蛋白质表达、细胞周期和细胞信号转导；4) 癌症功能基因组学核心提供切割- 用于癌细胞高通量活细胞功能表征的边缘技术和服务，包括反向蛋白质组学阵列建立、绝对基因定量和拷贝数测定、核酸酸和蛋白质电泳和成像、平板上高通量测定的多模式筛选、高通量、细胞中亚细胞定位蛋白的高内涵筛选、活细胞的代谢分析和癌细胞克隆形成测定集落的定量。 MBCR SR 实验室空间总计 6,409 平方英尺，位于 Stanton L. 的一楼、三楼和四楼。 OUHSC 校园内的青年生物医学研究中心 (BRC) 设施位于中心位置，提供了 27 个位于 BRC 的 SCC 成员可以立即访问这一重要的基础设施另外还有 5 个 SCC。成员位于相邻的生物医学科学大楼 (BMSB)，该大楼设有专门的内部办公室 2016 年，MBCR SR 用户总数的 22% 是经过同行评审的 SCC 成员。 2016 年，该机构还为 12 名 SCC 成员提供了未经同行评审的支持。占该集中式机构设施总用户的 32%，这是迄今为止使用量最大的机构 OUHSC 的部门或疾病现场小组，强调该设施的高利用率和影响 SCC 发起的一项激励计划涵盖了所有收费的 20%。 SCC 成员感谢 MBCR 所有领域提供的服务。