Characterisation of cortical subplate neurons

皮质下板神经元的表征

• 批准号: G0700377/1
• 负责人: Zoltan Molnar
• 金额: $ 87.07万
• 依托单位: University of Oxford
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0700377%2F1
• 关键词: Characterisation; cortical; subplate; neurons

Building the brain is like erecting a huge complex tower or bridge. The construction requires a dynamic scaffold which is built and modified along with the final permanent structure. After the completion of the construction the scaffold has to be dismantled at the right places and at the right time. The subplate, the earliest generated and largely transient cortical neurons is the foundation of the brain, and disruption of these cells may be the source of many developmental flaws, and therefore a fundamental topic to study. Cerebral cortical developmental disorders (schizophrenia, autism, attention deficit/hyperactivity disorder, ADHD) and perinatal hypoxic injuries (periventricular leucomalacia, PVL) involve cells of the subplate. The Molnar group has been investigating the integration of subplate cells into the intracortical and extracortical circuitry, their neurochemical properties and physiological characteristics over the last 15 years. The major hindrance of progress in the understanding of the role of this enigmatic cell population in cortical development and disease is the lack of reliable markers for these cells. In view of the selective vulnerability of this cell population and their important role in cortical development we propose to identify specific gene expression patterns in subplate and exploit this knowledge for further molecular and genetic approaches to study their role and vulnerability. These approaches require much specialised skills, which are beyond the scope of a single research group. The proposed collaboration will foster the interactions between a molecular and genetic laboratory and a cellular and anatomical group within the same Department of the same University and will open opportinities for collaboration with practising clinicians to initiate clinical applications in neuro-imaging, psychiatry, ophthalmology and neuropathology.

建立大脑就像竖立巨大的复杂塔或桥梁。该结构需要一个动态的脚手架，并与最终的永久结构一起构建和修改。建筑完成后，必须在正确的地方和正确的时间拆除脚手架。该子板是最早产生的，主要是短暂的皮质神经元的基础，这些细胞的破坏可能是许多发育缺陷的根源，因此是研究的基本话题。脑皮质发育障碍（精神分裂症，自闭症，注意力缺陷/多动障碍，ADHD）和围产期缺氧性损伤（周围性肾上腺素毛，PVL）涉及子板的细胞。在过去的15年中，Molnar组一直在研究亚板细胞中的整合到心脏内和心脏外回路，其神经化学特性和生理特征。在理解这种神秘的细胞种群在皮质发育和疾病中的作用方面进步的主要阻碍是这些细胞缺乏可靠的标记。鉴于该细胞种群的选择性脆弱性及其在皮质发育中的重要作用，我们建议在子板中识别特定的基因表达模式，并利用这种知识，以进一步的分子和遗传方法来研究其作用和脆弱性。这些方法需要许多专业技能，这超出了单个研究小组的范围。提出的合作将促进同一大学同一系中的分子和遗传实验室与细胞和解剖组之间的相互作用，并将与实践临床医生合作开放，以在神经图像，精神病学，眼科和神经病理学中启动临床应用。