Biomechanical Characterization of Human Cartilaginous End-Plate

人类软骨终板的生物力学特征

• 批准号: 7772312
• 负责人: Hai Yao
• 金额: $ 7.31万
• 依托单位: CLEMSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7772312
• 关键词: Address; Affect; Anisotropy; Behavior; Biochemical; Biomechanics; Blood Vessels; Cells; Charge; Country; Coupling; Data; Development; Diagnostic; Electric Conductivity; Elements; Environment; Equilibrium; Etiology; Extracellular Matrix; Goals; Health; Human; Human body; Hydration status; Individual; Intercellular Fluid; Intervertebral disc structure; Ions; Knowledge; Liquid substance; Low Back Pain; Measures; Mechanical Stress; Mechanics; Methods; Modeling; Nutrient; Nutritional; Pathway interactions; Permeability; Play; Property; Race; Research; Research Project Grants; Role; Route; Structure; Techniques; Testing; Theoretical model; Tissue Engineering; Tissues; United States; Wound Healing; abstracting; age effect; articular cartilage; density; insight; intervertebral disk degeneration; novel; nutrition; public health relevance; repaired; sex; socioeconomics; soft tissue; solute; theories; tissue regeneration; tool

DESCRIPTION (provided by applicant): Abstract Low back pain is a major socio-economic concern in the United States. Although the exact cause for low back pain is unclear, degeneration of the intervertebral disc (lVD) has been implicated as a possible primary etiologic factor. Poor nutritional supply is believed to be one of the mechanisms for disc degeneration and hampers any tissue engineering or repair attempt. Due to the unique composition and structure of the materials and the complexity of the mechano-electrochemical coupling phenomena in IVD tissues, there is a lack of knowledge on transport properties of human lVDs and appropriate theoretical models for investigating nutrient transport in IVD systematically. The goal of this research is to fill this gap by measuring transport properties of human IVD tissues, and develop a new mechano-electrochemical transport theory and finite element model for investigating the transport of fluid and solute in human IVD. The proposed study will focus on determination of the electromechanical and transport properties of human lumbar cartilaginous end-plate (CEP), which is thought to play an important role in disc nutrition and load distribution. Our hypothesis is that human CEP, compared to human articular cartilage, is stiffer and more permeable to the interstitial fluid and solutes, and mechanical loading affects the rates of fluid and solute transport in this tissue by changing tissue hydration. Two specific aims will be pursued during this study. Specific Aim #1 is to determine hydraulic permeability, fixed charge density, and electrical conductivity of human CEP under various mechanical strains, obtain ion diffusivities from electrical conductivity data, and develop new constitutive relationships between transport properties (hydraulic permeability and solute diffusivity) and tissue hydration to establish strain-dependent transport properties. Specific Aim #2 is to determine the compressive and shear mechanical properties of human CEP and correlate the material properties to the tissue composition. The obtained material properties will be used in developing a new multiphasic finite element model of human IVD to systematically quantify the physicochemical environment within the disc under various loading conditions. The advance in theory, numerical tools, data on material properties, and measuring techniques will have a significant impact on understanding etiology of disc degeneration as well as on developing new strategies for tissue regeneration. PUBLIC HEALTH RELEVANCE: Project Narrative The goal of this project is to determine the mechanical and transport properties of human cartilaginous end- plate and investigate the effect of mechanical strain on the fluid and solute transport for understanding the nutrition-related mechanism of intervertebral disc degeneration.

描述（由申请人提供）： 抽象的下背痛是美国的主要社会经济问题。尽管腰痛的确切原因尚不清楚，但椎间盘（LVD）的变性已被视为可能的主要病因因子。据信营养供应不足是椎间盘退化的机制之一，并阻碍了任何组织工程或修复尝试。由于材料的独特组成和结构以及IVD组织中机械 - 电化学偶联现象的复杂性，因此缺乏对人LVD的运输特性的知识以及适当的理论模型，用于系统地研究IVD中的营养转运。这项研究的目的是通过测量人类IVD组织的运输特性来填补这一空白，并开发一种新的机械电化学转运理论和有限元模型，以研究人类IVD中流体和溶质的运输。拟议的研究将着重于确定人类腰部软骨端板（CEP）的机电和运输特性，该特性在椎间盘营养和负载分布中起着重要作用。我们的假设是，与人类关节软骨相比，人类CEP对间质液和溶质更加渗透，并且更可渗透，机械负荷会通过改变组织水合来影响该组织中的液体和溶质转运速率。在本研究中将追求两个具体的目标。具体目的1是确定人CEP在各种机械菌株下的水力通透性，固定电荷密度和电导率水合以建立应变依赖性转运特性。具体目的＃2是确定人CEP的压缩和剪切机械性能，并将材料特性与组织组成相关。所获得的材料特性将用于开发人类IVD的新的多相有限元模型，以系统地量化各种载荷条件下的椎间盘内物理化学环境。理论上的进步，数值工具，材料特性数据和测量技术将对椎间盘退化的病因以及制定新的组织再生策略产生重大影响。 公共卫生相关性： 项目叙述该项目的目的是确定人类软骨端板的机械和运输特性，并研究机械应变对液体和溶质传输的影响，以理解椎间盘椎间盘变性的营养相关机理。

项目成果

Diffusivity of Human Cartilage Endplates in Healthy and Degenerated Intervertebral Disks.

健康和退化椎间盘中人体软骨终板的扩散性。

• DOI: 10.1115/1.4056871
• 发表时间: 2023
• 期刊: Journal of biomechanical engineering
• 作者: Ren,Pengling;Chen,Peng;Reeves,RussellA;Buchweitz,Nathan;Niu,Haijun;Gong,He;Mercuri,Jeremy;Reitman,CharlesA;Yao,Hai;Wu,Yongren
• 通讯作者: Wu,Yongren