Animal Core

动物核心

• 批准号: 7798888
• 负责人: JOHN P MANIS
• 金额: $ 36.06万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-12-01 至 2014-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7798888
• 关键词: Algorithms; Animal Model; Animals; Blood Cells; Breeding; Complex; Computer software; Core Facility; Engineering; Gene Expression; Generations; Genotype; Goals; House mice; Housing; Methods; Mouse Strains; Mus; Mutant Strains Mice; Regimen; Research Personnel; Resources; Tissues; Transgenic Mice; Transplantation; base; cost; cost effective; experimental analysis; granite; human disease; programs; promoter; tool

1.1 Introduction The principal goal of the Experimental Animal Core is to house mice in the more cost effective and efficient manner in a central facility for all three of the proposed projects. The generation of transgenic mice has proven to be a very powerful tool in answering biologic questions, especially those directly relevant to human disease. However, animal modeling has become increasingly complex with many variables to consider including mouse strain, the selection of tissue specific promoters to modulate targeted gene expression, as well as overall expense. The function of Core B is to serve as a centralized resource to house and maintain the animals and to keep the overall costs of experimental animals to a minimum for the program. In addition. Dr. John Manis will act as a scientific resource to the investigators in planning for the generation of compound mutant mice with multiple genotypes for experimental analysis. Table 1 lists the various genetically engineered animals and normal control mouse strains that will be employed in the scientific studies of the program, housed in the core facility, and used during the course of this proposal. Each mouse strain will be housed and generated in direct proportion to the need ofthe investigator, with a breeding and management algorithm using a specialized software based method (Granite).

1.1 简介 实验动物核心的主要目标是以更具成本效益和效率的方式将小鼠安置在所有三个拟议项目的中央设施中。转基因小鼠的产生已被证明是回答生物学问题的一个非常强大的工具，特别是那些与人类疾病直接相关的问题。 然而，动物建模变得越来越复杂，需要考虑许多变量，包括小鼠品系、组织特异性启动子的选择以调节目标基因表达以及总体费用。 核心 B 的功能是作为安置和维护动物的集中资源，并将实验动物的总体成本保持在最低限度。此外。 John Manis 博士将作为研究人员的科学资源，规划生成具有多种基因型的复合突变小鼠以进行实验分析。表 1 列出了将用于该计划科学研究、安置在核心设施中并在本提案过程中使用的各种基因工程动物和正常对照小鼠品系。每个小鼠品系将根据研究人员的需要直接按比例饲养和产生，并使用基于专门软件的方法（Granite）进行育种和管理算法。