Molecular determinants of nodaviral assembly

结病毒组装的分子决定因素

• 批准号: 7217551
• 负责人: Anette Schneemann
• 金额: $ 39.66万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-08-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217551
• 关键词: Address; Biochemical Genetics; Biological Models; Capsid Proteins; Electron Microscopy; Elements; Family; Fluorescence Microscopy; Genome; Housing; Life Cycle Stages; Molecular; Physical condensation; Process; RNA; RNA Viruses; Role; Viral Genome; Virion; Virus; Virus Assembly; Virus Diseases; particle; research study; viral RNA; virology

DESCRIPTION (provided by applicant): This proposal outlines experiments to develop a better understanding of icosahedral RNA virus assembly. It focuses on specific interactions between capsid protein and viral RNA as well as aspects that control recognition, condensation and encapsidation of the viral genome. It addresses the unresolved issue of how multipartite RNA genomes are recognized and packaged into a single virion and it seeks to define the role of cellular parameters in this process. The experimental plan takes advantage of a highly developed model system represented by the icosahedral RNA containing nodaviruses. Nodaviruses are a family of genetically, biochemically and structurally exceptionally well characterized viruses that have proven to be highly tractable for the study of fundamental questions in virology, including particle assembly and packaging of the genome. The specific aims of this project are: (1) to investigate the role of subcellular compartmentalization in the Flock House Virus (FHV) life cycle using biochemical and genetic approaches; (2) to visualize cellular compartmentalization and spatial dynamics during FHV infection by fluorescence microscopy and electron microscopy; (3) to identify mechanistic strategies and molecular elements involved in packaging of the FHV genome and (4) to define the role of FHV coat protein in folding and condensation of viral RNA during assembly.

描述（由申请人提供）：该提案概述了旨在更好地理解二十面体 RNA 病毒组装的实验。 它重点关注衣壳蛋白和病毒 RNA 之间的特定相互作用，以及控制病毒基因组识别、浓缩和衣壳化的方面。 它解决了如何识别多部分 RNA 基因组并将其包装成单个病毒体的未解决问题，并试图定义细胞参数在此过程中的作用。该实验计划利用了以含有诺达病毒的二十面体RNA为代表的高度发达的模型系统。 诺达病毒是一类在遗传、生物化学和结构上都具有非常明确特征的病毒，已被证明非常适合研究病毒学的基本问题，包括颗粒组装和基因组包装。 这 该项目的具体目标是：（1）研究亚细胞区室化在 使用生化和遗传方法研究羊群病毒（FHV）生命周期； (2) 形象化 通过荧光显微镜和电子显微镜观察 FHV 感染过程中的细胞区室化和空间动态； (3) 确定参与 FHV 基因组包装的机制策略和分子元件，以及 (4) 确定 FHV 外壳蛋白在组装过程中病毒 RNA 折叠和浓缩中的作用。