Mechanisms of de novo membrane assembly

从头膜组装的机制

基本信息

批准号：
10578067
负责人：
Aaron M Neiman
金额：
$ 31.36万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-01-01 至 2026-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10578067
关键词：
Adaptor Signaling Protein Address Autophagosome Biochemical Biological Models Biological Process Bypass Cell physiology Cells Centrioles Cytology Cytoplasm D Cells Defect Disease Docking Encapsulated Endoplasmic Reticulum Eukaryotic Cell Event Experimental Genetics Fluorescence Generations Genetic Guanine Nucleotides Health Human Hydrophobicity Intracellular Membranes Lead Licensing Lipids Mediating Meiosis Membrane Membrane Fusion Microtubule-Organizing Center Modeling Molecular Mutation Nature Neurodegenerative Disorders Organelles Orthologous Gene Pathologic Pathway interactions Point Mutation Population Process Protein Family Proteins Reporting Role SNAP receptor Saccharomycetales Site Surface Syndrome System Techniques Testing Vesicle Yeasts cell behavior ciliopathy experimental study flexibility in vitro testing in vivo insight interest lipid transfer protein membrane assembly nervous system disorder protein complex protein purification rab GTP-Binding Proteins recruit spindle pole body target SNARE proteins vesicular SNARE proteins

项目摘要

7. PROJECT SUMMARY/ABSTRACT The ability to rearrange vesicular traffic to create new membrane compartments de novo is a fundamental cell biological process important for many aspects of cellular physiology and differentiation. Two common examples of this phenomenon are the creation of autophagosomes and the generation of the ciliary sheath. In both cases, vesicles coalesce on a protein substrate to create a small double membrane that subsequently expands into a distinct organelle. Defects in these processes are associated with a variety of diseases, highlighting the importance of this aspect of cellular physiology to human health. Prospore membrane formation during the process of sporulation in budding yeast is another example of a de novo membrane formation event. Prospore membrane formation occurs on the microtubule organizing center of the cells, analogous to the ciliary sheath in higher cells, and the prospore membrane expands to encapsulate cytoplasmic components, analogous to an autophagosome. These similarities are due to parallels at the molecular level that make yeast sporulation a powerful model system to identify the underlying molecular mechanisms of membrane assembly. The experiments in this proposal are focused on two aspects of this process: 1) how the MOP promotes coalescence of vesicles to create the prospore membrane, and 2) how the Vps13 lipid transfer protein promotes membrane expansion.

7. 项目概要/摘要重新排列囊泡交通以从头创建新的膜区室的能力是一种基本细胞生物过程对于细胞生理学和分化的许多方面都很重要。二这种现象的常见例子是自噬体的产生和睫状体的产生鞘。在这两种情况下，囊泡在蛋白质底物上结合形成一个小的双层膜，随后扩展成一个独特的细胞器。这些过程中的缺陷与多种相关疾病，强调了细胞生理学这一方面对人类健康的重要性。原生孢子芽殖酵母孢子形成过程中膜的形成是从头形成的另一个例子膜形成事件。原生孢子膜的形成发生在微管组织中心细胞，类似于高等细胞中的睫状鞘，并且前孢子膜扩张以包裹细胞质成分，类似于自噬体。这些相似之处是由于相似之处分子水平使酵母孢子形成成为识别潜在分子的强大模型系统膜组装机制。本提案中的实验主要集中在两个方面过程：1) MOP 如何促进囊泡聚结以形成原生孢子膜，以及 2) 如何 Vps13 脂质转移蛋白促进膜扩张。