Fast Optical Imaging of Cortical Signals in the Behaving Primate

行为灵长类动物皮层信号的快速光学成像

基本信息

批准号：
7295679
负责人：
ANNA W. ROE
金额：
$ 18.63万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-30 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7295679
关键词：
Animals Area Attention Behavior Behavioral Blood Vessels Brain imaging Characteristics Clinical Cognitive Compatible Complement Computer software Data Decision Making Detection Development Disease Disruption Dissociation Dyes Electrophysiology (science)Event Functional Imaging Functional Magnetic Resonance Imaging Funding Goals Image Image Analysis Imagery Imaging Techniques Imaging technology Impaired cognition Invasive Lead Link Location Macaca Maps Memory Methodology Methods Monitor Monkeys Nature Neurons Noise Numbers Optics Pattern Performance Physiological Physiology Prefrontal Cortex Primates Process Psyche structure Research Personnel Research Proposals Resolution Schizophrenia Short-Term Memory Signal Transduction Stream System Techniques Technology Time Training Vision Visual Work awake base clinically relevant cognitive function image processing improved memory process millisecond novel novel strategies oculomotor optical imaging programs relating to nervous system research study response sensory cortex somatosensory spatiotemporal technology development voltage

项目摘要

DESCRIPTION (provided by applicant): The goal of this research proposal is to develop a fast temporal resolution optical imaging methodology for use in awake, behaving monkeys. If successful, this methodology would provide online visualization of brain function at both high spatial (10's of ums) and high temporal (1-10 msec) resolution. Intense effort has been devoted to studying anatomical organization and physiological responses of sensory cortex in anesthetized and awake monkeys. However, little is known about the spatial patterns of activation in prefrontal cortex of awake, behaving animals during performance of working memory tasks. The goal is to observe clustered and distributed clusters of neuronal activation of cortical activity during behavior. These data would also provide a critical link between single-unit physiology, functional imaging studies, and behavioral studies. Importantly, the proposed technology development will provide new methodologies for studying spatial (functional organizational) and temporal aspects of cortical processing relating to vision, attention, memory, and cognitive function. Clinical relevance of data obtained from this approach would be relevant to cognitive dysfunctions in diseases such as schizophrenia. The specific aims of this proposal are: 1) development of fast optical imaging system for awake, behaving monkeys. This aim would include development of voltage sensitive dye methodology, noise reduction techniques for optical detection of neuronal activity, rapid image processing techniques suitable for large volumes of streamed data, and tandem electrophysiological recordings. 2) application of this method to study working memory processes in prefrontal cortex. Macaque monkeys would be trained on oculomotor delay response tasks. Delay period activity in prefrontal cortex would be imaged at high temporal (1-10 msec) to monitor changing spatiotemporal activity patterns depending on task demands. This methodology will provide a new approach for examining relationship of cortical organization with behavior, one that will complement the spatiotemporal capabilities of other existing technologies such as fMRI and single unit electrophysiology.

描述（由申请人提供）：这项研究计划的目标是开发一种快速时间分辨率光学成像方法，用于清醒、行为正常的猴子。如果成功，该方法将提供高空间（10 微米）和高时间（1-10 毫秒）分辨率的大脑功能在线可视化。人们致力于研究麻醉和清醒猴子感觉皮层的解剖结构和生理反应。然而，人们对清醒、行为动物在执行工作记忆任务时前额皮质激活的空间模式知之甚少。目标是观察行为过程中皮层活动的聚集和分布的神经元激活簇。这些数据还将提供单单位生理学、功能成像研究和行为研究之间的关键联系。重要的是，所提出的技术开发将为研究与视觉、注意力、记忆和认知功能相关的皮质处理的空间（功能组织）和时间方面提供新的方法。从这种方法获得的数据的临床相关性将与精神分裂症等疾病的认知功能障碍相关。该提案的具体目标是：1）为清醒、有行为能力的猴子开发快速光学成像系统。这一目标将包括开发电压敏感染料方法、用于神经元活动光学检测的降噪技术、适合大量流数据的快速图像处理技术以及串联电生理记录。 2）应用该方法研究前额叶皮层的工作记忆过程。猕猴将接受动眼神经延迟反应任务的训练。前额皮质的延迟期活动将在高时间（1-10 毫秒）进行成像，以根据任务需求监测时空活动模式的变化。该方法将为检查皮质组织与行为的关系提供一种新方法，该方法将补充其他现有技术（例如功能磁共振成像和单单元电生理学）的时空能力。