Electronic Holography for Biomedical Applications Using a Coherent Source

使用相干源的生物医学应用电子全息术

• 批准号: G0601733/1
• 负责人: Hongyue Sun
• 金额: $ 13.33万
• 依托单位: University of Aberdeen
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0601733%2F1
• 关键词: Electronic; Holography; Biomedical; Applications; Using

Observations of cell movement in three-dimension within living tissues is very important for the research in biomedical sciences. However, the link between the wealth of cell behavioral information from two dimensional cultures and the natural physiology and pathology is missing when biological samples are prepared for examination under light microscope in the laboratory . The proposal will combine the expertise in digital holography of physical sciences and that in cell movement in biomedical sciences at University of Aberdeen and build a digital holographic system to study living biological samples. This will allow us to visualize cell movement in its actual living environment, including fast moving cells, such as those in the blood stream, which is not currently possible to be observed at high resolution. A digital hologram of a living sample can be conveniently recorded on an electronic sensor and then reconstructed in a computer. The reconstructed holographic images show the original scene of the living samples of interests in three dimensional space. The new system provides extra information for examining living cell behaviours compare with the conventional imaging systems with two dimensional sampling mechanisms. At the University of Aberdeen, the optical engineering group has more than 25 years experience in optical engineering and is one of the leading groups in the world specializing in holographic imaging (Watson et al, Meas. Sci. Technol., vol. 12 pp L9-15, 2001, Sun et al, J. Opt. A: Pure Appl. Opt. 6 pp703-10, 2005) and have built one of the most advanced electronic holographic camera for marine biology. The Medical Sciences Group has more than ten years experience in cell migration study and have recently made a significant contribution in understanding cell movement in wound healing (Zhao et al, Nature, vol. 442, pp 457-60, 2006). The ?hopping? from optical engineering discipline to biomedical sciences is aiming to allow the applicant to gain extensive experience of working in biomedical sciences discipline and apply the optical engineering ideas of physical sciences to medical sciences effectively, and to build a digital/electronic holographic video microscopy system. It is expected that the hopping will significantly promote new interactions between groups of Optical Engineering and Medical Sciences at the University of Aberdeen.

对生物医学科学研究中三维的细胞运动观察非常重要。但是，当在实验室的光学显微镜下准备好检查生物样品时，从二维培养物中的大量细胞行为信息与自然生理和病理之间的联系之间存在联系。该提案将结合物理科学数字全息的专业知识，以及阿伯丁大学生物医学科学的细胞运动中的专业知识，并建立一个数字全息系统来研究生物学样品。这将使我们能够在其实际的生活环境中可视化细胞运动，包括快速移动的细胞，例如血流中的细胞，目前无法在高分辨率下观察到这些细胞。可以在电子传感器上方便地记录活样品的数字全息图，然后在计算机中重建。重建的全息图像显示了三维空间中感兴趣的生存样本的原始场景。新系统提供了额外的信息，用于检查活细胞行为与具有二维采样机制的常规成像系统相比。在阿伯丁大学，光学工程组在光学工程领域拥有超过25年的经验，并且是全球领先的小组之一，专门从事全息成像（Watson等人，Meas。Sci。Technol。，第12 pp l9-15，2001，Sun等，Sun等，J。Opt。A：PureAppl。A：Pure appl.6 Pp703-103-103-10，2005年，均为2005年，extronic for. 6 pp703-105），海洋生物学。医学科学组在细胞迁移研究方面拥有十多年的经验，最近在理解伤口愈合的细胞运动方面做出了重大贡献（Zhao等，《自然》，第442卷，第457-60页，2006年）。跳跃？从光学工程学科到生物医学科学，旨在使申请人获得在生物医学科学学科工作的丰富经验，并将物理科学的光学工程思想应用于医学科学，并建立数字/电子全息视频显微镜系统。预计跳跃将大大促进阿伯丁大学光学工程和医学科学组之间的新互动。