Core--Animal Research

核心--动物研究

• 批准号: 7846895
• 负责人: AAMIR Rafiq ZUBERI
• 金额: $ 19.13万
• 依托单位: LSU PENNINGTON BIOMEDICAL RESEARCH CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7846895
• 关键词: Affect; Animal Experimentation; Animal Model; Biomedical Research; Botanicals; Classification; Clinical Research; Data Analyses; Diet; Experimental Models; Future; Genotype; Goals; Grant; Inbred Strains Mice; Laboratories; Metabolic syndrome; Molecular Genetics; Mouse Strains; Phase; Phenotype; Phytochemical; Regulation; Research; Research Personnel; Role; Screening procedure; Signal Pathway; Testing; Transgenic Mice; Upper arm; Work; design; dosage; in vivo; mouse model; mutant; research study; therapy development

The characterization of botanical action on the regulation of the metabolic syndrome has been hampered by a lack of systematic approaches in understanding the full extent of affected phenotypes and in fully considering the effects of botanical-diet and botanical-genotype interactions. The Animal Research Core (ARC) will function as an in vivo phenotyping and research arm of the botanical research center using mouse models of metabolic syndrome as an experimental model for biomedical research. The goal of the ARC is to identify key signaling pathways in the development and treatment of the metabolic syndrome that are affected by botanical action, and to work closely with the Botanical Core to test and identify key phytochemical(s) that promote these changes. We will achieve this goal by following three specific aims. One, to work closely with the Center Grant investigators to help design, perform and help analyze data from the animal research phase of the specific projects, and to develop appropriate dosage and toxicological screening to facilitate downstream future clinical studies. Two, because the experimental paradigms differ between the projects, we will develop and utilize a systematic screening and phenotyping approach to facilitate the comparison of different botanicals on the development and treatment of the metabolic syndrome, and to screen for and characterize new and previously unknown new phenotypes affected by botanical action. This will include a characterization of botanical action in the context of defined and differing diets. Three, to take advantage of the classical and molecular genetic expertise of the ARC Project Leader, to design experiments to characterize botanical-genotype interactions in different inbred mouse strains. A major role for the ARC will be to functionally test hypotheses about botanical mechanism of action that emerge from the core investigators laboratories in animal models. We will use specific mutant and transgenic mouse strains that are specifically perturbs in the candidate signaling pathways to confirm or refute the stated hypotheses.

缺乏系统的方法在理解影响表型的全部程度以及充分考虑植物 - 植物和植物学型相互作用的影响方面缺乏系统的方法，从而阻碍了植物作用对代谢综合征调节的表征。动物研究核心（ARC）将使用代谢综合征的小鼠模型作为一个体内表型和研究中心的研究部门 生物医学研究的实验模型。 ARC的目的是确定受植物作用影响的代谢综合征的开发和处理中的关键信号通路，并与植物核心紧密合作，以测试和识别促进这些变化的关键植物化学化学物质。我们将遵循三个具体目标来实现这一目标。第一，与中心赠款调查人员紧密合作，以帮助设计，执行和帮助分析特定项目动物研究阶段的数据，并开发适当的剂量和毒理学筛查，以促进下游未来的临床研究。第二，由于项目之间的实验范例有所不同，我们将开发和利用系统的筛查和表型方法来促进不同植物因素在代谢综合征开发和处理方面的比较，并筛查筛查以进行筛查以进行筛查 并表征受植物作用影响的新的和以前未知的新表型。这将包括在定义和不同饮食的背景下对植物作用的特征。第三，要利用ARC项目领导者的经典和分子遗传专业知识，以设计实验以表征不同近交小鼠菌株中植物性型相互作用。 ARC的主要作用将是在功能上检验有关动物模型中核心研究人员实验室的植物作用机理的假设。我们将使用特定的突变体和转基因小鼠菌株，这些菌株是特定的候选信号通路中的杂物，以确认或反驳所指定的假设。