Mouse Core

鼠标核心

• 批准号: 8011759
• 负责人: WILLIAM H. MCBRIDE
• 金额: $ 40.34万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-05 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8011759
• 关键词: Address; Animal Experimentation; Animal Experiments; Animal Model; Animals; Biohazardous Substance; Breeding; Cesium; Cobalt; DNA; DNA Repair; Ensure; Experimental Designs; Funding; Gene Transfer; Genetic; Gnotobiotic; Guidelines; Health; IACUC; Immune; Immunity; Individual; Maintenance; Methods; Modeling; Monitor; Mus; Normal tissue morphology; Pathway interactions; Performance; Pilot Projects; Procedures; Quality Control; Radiation; Radioisotopes; Research; Research Personnel; Research Project Grants; Resources; Roentgen Rays; Services; Source; Tissues; Toll-like receptors; Training; Transgenic Mice; Transgenic Organisms; base; cost; cytokine; design; irradiation; member; pathogen; radiation effect; research study; response; success

The UCLA-CMCR Animal Core provides a full support fro animal experiments in all Full Projects and many Pilot Projects. The Core is a self-contained, fiscally independent, strict barrier facility that breeds and maintains, at a low cost, the highest possible quality gnotobiotic mice with a defined microbiological flora. It maintains breeding colonies of multiple immune competent, immune deficient, and transgenic strains that allow definitive studies on DNA repair, DNA damage response, cytokine, Toll-like receptor and other pathways that are relevant to radiation effects. In the past funding period, an average of 5,500 mice per year were used by full projects and it contributed greatly to the success of the UCLA-CMCR. The facility has an AEC cesium source 'en suite' with jigs enabling whole and partial body irradiations to be performed. X-ray and cobalt radiation machines are also available. It provides assistance with animal Irradiations on an as needed basis. It has a level II Biohazard facility and can accommodate studies on gene transfer, radioisotopes, and pathogens and provide assistance with such studies. The Core assists investigators in obtaining IACUC approval, monitors compliance and animal health, as well as providing training and advice for investigators in animal handling, procedures, the design and performance of radiation experiments and analysis and interpretation of results. This resource is available to all members of the UCLA-CMCR and others outside of UCLA on a recharge basis. These activities can be fully integrated because the facility is under the direct control of Dr. McBride who has the power to allocate space and resources to the CMCR, whose investigators receive priority. The Core brings great expertise in the responses of most normal tissues to radiation, in DNA repair, in genetics, and in immunity. It provides a variety of models to address the product potential of mitigators of radiation tissue damage and the pathways by which they may operate. It will perform a vital function in integrating the projects within the CMCR and in standardizing the information that is derived from individual animal experimentation. In so doing, it will give added value to the CMCR.

UCLA-CMCR动物核心在所有完整项目中提供了全方位的支持，许多项目和许多 试点项目。核心是一种独立，独立，严格的障碍设施，繁殖和 以低成本维持具有定义的微生物菌群的最高质量gnotobiotic小鼠。它 维持多种免疫，免疫缺陷和转基因菌株的繁殖菌落， 允许对DNA修复，DNA损伤反应，细胞因子，Toll样受体和其他的确切研究 与辐射效应相关的途径。在过去的资金期间，平均每年有5500只小鼠 被完整的项目使用，它为UCLA-CMCR的成功做出了巨大贡献。 该设施具有带有夹具的AEC Cesium源为“连接套房”，使整体和部分的照射能够 执行。还提供X射线和钴辐射机。它为动物提供帮助 根据需要进行辐照。它具有II级生物危害设施，可以容纳有关基因的研究 转移，放射性同位素和病原体，并通过此类研究提供帮助。核心协助 研究人员获得IACUC批准，监视合规性和动物健康以及提供 为调查人员的动物处理，程序，辐射设计和性能的培训和建议 实验和分析和结果的解释。此资源可用于所有成员 UCLA-CMCR和加州大学洛杉矶分校以外的其他人以充电为基础。这些活动可以完全集成 因为该设施受到麦克布赖德博士的直接控制，他有权分配空间， CMCR的资源，其调查人员获得了优先级。 核心在大多数正常组织对辐射的反应，在DNA修复中，在 遗传学和免疫力。它提供了各种模型来解决缓解剂的产品潜力 辐射组织损伤及其可能操作的途径。它将在 将项目集成到CMCR中，并在标准化个人的信息中标准化 动物实验。这样一来，它将为CMCR提供附加值。