CHEMICAL MECHANISMS OF ENZYMES

酶的化学机制

• 批准号: 7953881
• 负责人: THOMAS JOSEPH KAPPOCK
• 金额: $ 0.12万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7953881
• 关键词: Amaze; Area; Cells; Chemicals; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Environment; Enzymes; Funding; Grant; Institution; Learning; Mass Spectrum Analysis; Metabolic Pathway; Molecular Biology; Reaction; Regulation; Research; Research Personnel; Resources; Side; Source; Transition Elements; United States National Institutes of Health; Work; aqueous; biomedical resource; cofactor; cold temperature; enzyme mechanism; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reactions carried out by enzymes occur at comparatively low temperatures, usually at near-neutral pH in an aqueous environment, typically with 100% yields and 100% ee's. Enzymes almost never make undesired side products, which can be attributed to an amazing level of control over the desired reaction. This area of research asks quantitative questions about enzymes and metabolic pathways, in hopes of learning how enzymes perform difficult chemical transformations and respond to hostile environments. Enzymes that use transition metals as cofactors are of special interest. While the projects focus on enzyme mechanism and regulation in cells, experimental approaches are drawn from diverse areas of chemistry and molecular biology. The projects afford many opportunities for collaborative work.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 通过酶进行的反应在相对较低的温度下发生，通常在水性环境中近乎中性pH值，通常为100％的产率和100％EE。 酶几乎永远不会生产不需要的侧产品，这可以归因于对所需反应的惊人控制水平。 该研究领域提出了有关酶和代谢途径的定量问题，以期了解酶如何进行艰难的化学转化并对敌对环境做出反应。 将过渡金属用作辅助因子的酶具有特殊的兴趣。 尽管这些项目着重于细胞中的酶机制和调节，但实验方法是从化学和分子生物学领域的不同领域提取的。 这些项目为协作工作提供了许多机会。