MS FOR PROBING RESISTANCE STRATEGIES IN ACETIC ACID BACTERIA

用于探测乙酸细菌耐药策略的 MS

• 批准号: 8361379
• 负责人: THOMAS JOSEPH KAPPOCK
• 金额: $ 1.62万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361379
• 关键词: Acetic Acids; Acetobacter; Acids; Bacteria; Biochemical; Cell membrane; Cellular Structures; Chemicals; Drops; Environment; Enzymes; Funding; Grant; Individual; Life; Mass Spectrum Analysis; Metabolic; Microbe; National Center for Research Resources; Organism; Plants; Principal Investigator; Production; Proteins; Research; Research Infrastructure; Resistance; Resources; Source; United States National Institutes of Health; Vinegar; X-Ray Crystallography; bacterial resistance; biological adaptation to stress; biomedical resource; coping; cost; dimer; genome sequencing; pathogen; resistant strain

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Bacteria cope with life in harsh environments using specific stress responses and chemical alterations of cell components. We explore the biochemical strategies for survival at low pH employed by a naturally acid-resistant bacterium, Acetobacter aceti, which has the ability to handle low pH in general -- its cytoplasmic pH can drop as low as 4 -- and acetic acid in particular. This Gram-negative, plant-associated bacterium has been used for millenia in the production of vinegar. Acetic acid is especially toxic to bacteria at low pH because it forms dimers that easily permeate cell membranes. Kappock's approach to this problem is centered on enzyme function at low pH, assisted by several in-progress genome sequencing projects. We found that individual A. aceti enzymes retain function in acid much longer than comparable forms from non-acidophilic organisms. X-ray crystallography has revealed distinctive architectural features of A. aceti proteins that may confer this stability. We have also delineated metabolic strategies for acetic acid elimination by highly-resistant strains, which may be employed by other microbes including several pathogens.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 细菌使用特定的压力反应和细胞成分的化学改变来应对恶劣环境中的生活。我们探索了由天然抗酸性细菌，乙酰杆菌乙酰乙酸具有低pH值的生存策略，该抗酸乙酸具有处理低pH值的能力 - 其细胞质pH值可能低至4-乙酸，尤其是乙酸。这种革兰氏阴性的，与植物相关的细菌已用于生产醋。乙酸在低pH值下对细菌特别有毒，因为它形成了容易渗透细胞膜的二聚体。 Kappock的此问题方法以低pH值的酶功能为中心，并在几个过程内基因组测序项目的帮助下。我们发现，单个乙酰曲霉的酶在酸中保留的功能比非酸性生物的可比形式长得多。 X射线晶体学揭示了可能赋予这种稳定性的A. aceti蛋白的独特建筑特征。我们还通过高度抗性菌株描绘了消除乙酸的代谢策略，这些菌株可能由包括几种病原体在内的其他微生物使用。