Raman spectroscopic evaluation of protein modification: a new, non-invasive diagnostic tool for age-related eye disease

蛋白质修饰的拉曼光谱评估：一种针对年龄相关眼病的新型非侵入性诊断工具

• 批准号: G0600053/1
• 负责人: Alan Stitt
• 金额: $ 55.19万
• 依托单位: Queen's University Belfast
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=G0600053%2F1
• 关键词: Raman; spectroscopic; evaluation; protein; modification

Rapid, non-invasive evaluation of robust markers of disease progression in the eyes of aging patients would provide a novel and exciting basis for recognising risk of visual loss and perhaps also link to other age-related diseases such as cataract formation and glaucoma. The proposed project builds on knowledge from the scientific literature, strong international collaboration and key preliminary findings. It will seek to establish a firm basis for using confocal Raman microscopy and spectroscopic evaluation of patients to study chemical modifications called advanced glycation endproducts (AGEs) in the eye. The approach is multidisciplinary and represents collaboration between vision scientists, physical chemists, ophthalmologists and optometrists. Together, this unusual team can offer a unique perspective on a pressing research problem. The project will ascertain age-related risk in a patient-based evaluation of AGE-mediated Raman spectra in ocular tissues. With appropriate identification, quantification and validation of AGE moieties in the eye and their correlation with age-mediated pathophysiology, Raman spectra could ultimately form an important, non-invasive diagnostic tool for AMD and other age-associated ocular defects.

衰老患者眼中疾病进展的鲁棒标记的快速，无创的​​评估将为识别视觉丧失的风险提供新颖而令人兴奋的基础，并可能与其他与年龄有关的疾病（例如白内障形成和青光眼）有联系。拟议的项目以科学文献，强大的国际合作和关键初步发现为基础。它将寻求建立一个牢固的基础，以使用患者的共聚焦拉曼显微镜和光谱评估研究，以研究眼睛中称为晚期糖基化终产物（AGE）的化学修饰。该方法是多学科的，代表了视觉科学家，物理化学家，眼科医生和验光师之间的合作。这支不寻常的团队共同可以就紧迫的研究问题提供独特的观点。该项目将在基于患者的眼部组织中对年龄介导的拉曼光谱的评估中确定与年龄有关的风险。通过适当鉴定，对眼睛中的年龄部分的定量和验证及其与年龄介导的病理生理学的相关性，拉曼光谱最终可能会形成一个重要的，无创的诊断工具，用于AMD和其他年龄相关的眼部缺陷。