CORE--MOUSE

核心--鼠标

基本信息

批准号：
7901824
负责人：
Lei Wei
金额：
$ 35.85万
依托单位：
INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-05-01 至 2012-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7901824
关键词：
Alleles Animal Husbandry Animals Biopsy Breeding Budgets Cell Maintenance Cells Charge Chimera organism Chloroform Collection Cultured Cells DNA Diagnostic Digestion Doctor of Philosophy ES Cell Line Electroporation Embryo Endopeptidase K Exons Expenditure Female Filtration Fostering Freezing Gel Generations Genes Genetic Genetically Modified Animals Genome Genotype Germ Lines Glass Harvest Heart failure Human Chorionic Gonadotropin Human Resources Hyaluronidase Indiana Injection of therapeutic agent International Unit Knock-out Knockout Mice Laboratories Lead Maintenance Mammalian Oviducts Microinjections Modeling Mus Neonatal Nuclear Participant Partner in relationship Phenols Pregnancy Production Program Research Project Grants Publishing Reagent Relative (related person)Reliance Research Personnel Resistance Resources Restriction fragment length polymorphism Sampling Schedule Sepharose Serum Services Signal Transduction Southern Blotting Staging Sterility Stress Structure Tail Time Tissues Training Transfection Transgenes Transgenic Model Transgenic Organisms Universities Vagina Weaning animal colony antibiotic G 418 blastocyst cell bank cost cost effective embryonic stem cell experience homologous recombination male mouse model pregnant programs pup research study success transmission process vector

项目摘要

Core B is the Genetically Modified Mouse Resources Core. All three projects will make extensive use of genetically modified mice for their proposed experiments. Centralized localization of the animals, rational control and sharing of the breeding stocks, and centralization of genotype analyses will maximize efficiency and minimize costs associated with the production of the mice needed for the propose experiments. Specific activities of Core B include (1) Assistance in the generation of additional genetically modified mouse models, (2) Establishment and maintenance of breeding stocks, (3) Generation and genotype analysis of transgenic and/or knock-out mice, (4) Generation and collection of timed pregnancies, (5) Fostering, weaning and storage of the experimental animals, (6) Distribution of genetically modified mouse models, and (7) Delivery of experimental animals to the appropriate laboratory for use. Core personnel are already well-trained in all aspects of the services to be provided. Given the heavy reliance on genetically modified animals, the importance of Core B to the success of the Program Project Grant application cannot be overstated.

核心B是转基因小鼠资源核心。这三个项目都将广泛利用转基因小鼠用于他们提出的实验。动物集中定位，合理种畜的控制和共享以及基因型分析的集中化将最大限度地提高效率并最大限度地减少与所提议的实验所需的小鼠的生产相关的成本。具体的核心 B 的活动包括 (1) 协助生成额外的转基因小鼠模型， (2)种畜的建立和维持，(3)转基因的产生和基因型分析和/或基因敲除小鼠，(4) 定时妊娠的产生和收集，(5) 寄养、断奶和实验动物的储存，（6）转基因小鼠模型的分发，以及（7）交付将实验动物送往适当的实验室使用。核心人员均已接受过良好的培训所提供服务的各个方面。鉴于对转基因动物的严重依赖，核心 B 对计划项目补助金申请成功的重要性怎么强调也不为过。