FLOW CYTOMETRY

流式细胞术

• 批准号: 7843304
• 负责人: Anne I. Sperling
• 金额: $ 16.47万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7843304
• 关键词: Address; Advisory Committees; Area; Arts; Basic Science; Cancer Center; Cancer Center Support Grant; Cell Separation; Cell physiology; Cells; Consultations; Continuing Education; Detection; Development; Educational workshop; Equipment and supply inventories; Faculty; Flow Cytometry; Fluorescence; Fractionation; Goals; Human Resources; Libraries; Measures; Methods; Mission; Molecular; NCI Center for Cancer Research; Operative Surgical Procedures; Research Personnel; Sampling; Services; Speed; Suspension substance; Suspensions; Technology; Training; Training and Education; University of Chicago Cancer Research Center; Work; design; improved; instrument; instrumentation; meetings; member; programs; protocol development

The Flow Cytometry Facility serves the UCCRC members by providing access to state-of-the-art technology, and quantitative analytical approaches to measure molecular and cellular function. The Facility is designed to meet the wide-spread needs for specialized cytologic analysis, and continues to respond to the demand for new and improved technology. The Facility's mission is three-fold: 1) To provide cutting-edge technology and expertise; 2) To pursue technology and application development; and 3) To provide education and training of researchers. The first goal is attained with state-of-the-art instrumentation and continuing education for the expert personnel. Technology and application development involves close relationships with Cancer Research Center researchers who are continually looking for new methods to address their scientific questions. Educational goals are satisfied by providing application workshops, training sessions, and one-on-one protocol development and consultation. The Facility also provides an extensive reference library containing references pertinent to its technological focus. The Facility's Scientific Director (Dr. Anne Sperling) and Technical Director (Mr. Ryan Duggan) provide direct oversight of the operation of the Flow Cytometry Facility. A Faculty Advisory Committee (FAC) is advisory to the Facility personnel with regards to services, scientific direction, and fiscal issues. The Facility is utilized by UCCRC investigators in many areas of study involved in the basic research of cellular function. The Flow Cytometry Facility provides instrumentation for the analytical detection of subcellular components using multiparametric fluorescence detection technology. Another key component of the Facility is its cell sorting instruments that allow the fractionation and purification of subpopulations of cells from a heterogeneous single cell suspension. The Flow Cytometry Facility has increased its cell sorting and bench-top analyzer capacity to meet investigator demand, resulting in more than a 300% increase in overall Facility usage since the 2001 CCSG renewal. Over 52 peerreviewed UCCRC investigators across five Scientific Programs routinely use the Flow Cytometry Facility, totaling 80% of Facility usage.

流式细胞术设施通过提供最先进的技术为 UCCRC 成员提供服务 技术和定量分析方法来测量分子和细胞功能。这 该设施旨在满足专业细胞学分析的广泛需求，并继续 响应对新技术和改进技术的需求。该设施的使命有三重： 1）提供尖端技术和专业知识； 2）追求技术和应用开发；和 3) 提供研究人员的教育和培训。 第一个目标是通过最先进的仪器和专家的继续教育来实现的 人员。技术和应用开发涉及与癌症的密切关系 研究中心的研究人员不断寻找新方法来解决他们的科学问题 问题。通过提供应用研讨会、培训课程和 一对一的协议制定和咨询。该设施还提供了广泛的参考 包含与其技术重点相关的参考资料的库。该设施的科学主任（博士） Anne Sperling）和技术总监（Ryan Duggan 先生）直接监督 流式细胞术设施。教员咨询委员会（FAC）为设施人员提供咨询 关于服务、科学方向和财政问题。 UCCRC 研究人员在涉及基础研究的许多研究领域中使用该设施 细胞功能。流式细胞术设施提供用于分析检测的仪器 使用多参数荧光检测技术检测亚细胞成分。另一把钥匙 该设施的组成部分是其细胞分选仪器，可对细胞进行分馏和纯化 来自异质单细胞悬浮液的细胞亚群。流式细胞术设施有 增加了细胞分选和台式分析仪的能力以满足研究人员的需求，从而 自 2001 年 CCSG 更新以来，总体设施使用量增加了 300% 以上。超过 52 个同行评审 UCCRC 五个科学项目的研究人员经常使用流式细胞术 设施，总计占设施使用量的 80%。