ANIMAL BARRIER SHARED RESOURCE

动物屏障共享资源

基本信息

批准号：
7886683
负责人：
LAWRENCE H HERBST
金额：
$ 28.07万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7886683
关键词：
Address Albert Einstein Cancer Center Alleles Animal Experimentation Animal Husbandry Animal Technicians Animal Welfare Animals Antineoplastic Agents Area Arts Behavioral Biopsy Breeding Cancer Center Cancer Center Support Grant Caring Censuses Characteristics Comprehensive Health Care Diagnostic Services Doctor of Philosophy Doctor of Veterinary Medicine Environment Faculty Film Floor Genetic Genetic Predisposition to Disease Genotype Gnotobiotic Goals Grant Growth Health Histopathology House mice Housing Implant Individual Infectious Agent Institutes Laboratory Animals Mission Modeling Monitor Mus Mutation Neoplasm Metastasis Neoplasms Pathologist Pathology Phenotype Price Process Quarantine Research Research Personnel Resource Sharing Resources Rodent Services Site Visit Specific Pathogen Frees Technology Testing Time Tissues Training Transgenes Tumor Tissue Veterinarians angiogenesis animal care animal resource flexibility germ free condition high standard improved novel programs quality assurance research study translational medicine tumor growth tumor xenograft

项目摘要

The Animal Barrier Shared Resource (ABSR) supports the mission of the AECC by providing an environment in which the highest quality of animal research studies can be conducted while maintaining the highest standards of animal care and animal welfare. A major objective of the ABSR is to maintain the health quality of barrier housed specific pathogen-free (SPF) mice, keeping them free of adventitious murine infectious agents that could disrupt breeding or confound research results. Barrier housing is provided for SPF mice while permitting supervised access for investigators. AECC investigators maintain breeding colonies either to expand novel strains bearing transgene or targeted mutations from genetically-manipulated founder animals, or to recombine several specific alleles from different genetic loci into a limited set of genotypes on a single strain background. Frequent convenient access is crucial for investigators so that they may set up matings, collect tissue biopsies from successive litters for genotype determination, and observe animals for physical and behavioral characteristics (phenotype), specifically, their propensity to develop neoplasia. In addition, many AECC investigators use specific pathogen-free rodents that either have a genetic predisposition to develop spontaneous neoplasia or are immunodeficient and tolerate tumor xenografts as models to study specific processes such as angiogenesis and metastasis and for testing antineoplastic therapies. These investigators must have frequent access to their animals to implant tumor tissues, monitor tumor growth, administer various treatments, and collect tissue biopsies. Animal husbandry services, veterinary care, and comprehensive rodent quality assurance and quarantine programs are provided to the AECC by the Institute of Animal Studies (IAS). The IAS consists of three faculty Laboratory Animal Veterinarians (including the Director), three Veterinary Technicians, a staff of approximately 40 Animal Caretakers, a Facilities Manager, two Husbandry Supervisors, as well as office administrative staff. In addition to providing veterinary care, the IAS veterinarians also provide oversight of the animal care and use program, monitor regulatory compliance, and conduct small-group and individual training sessions for investigators. Pathology diagnostic services are provided by a Veterinary Pathologist who also directs the Histopathology Shared Resource.

动物屏障共享资源 (ABSR) 通过提供可以进行最高质量的动物研究的环境，同时保持动物护理和动物福利的最高标准。 ABSR 的一个主要目标是维持屏障饲养的无特定病原体 (SPF) 小鼠的健康质量，使它们免受外来鼠害可能破坏繁殖或混淆研究结果的传染性病原体。提供屏障外壳用于 SPF 小鼠，同时允许研究人员在监督下进入。 AECC 调查人员维持育种菌落要么用于扩展带有转基因的新菌株，要么来自基因操作的靶向突变创始人动物，或将来自不同遗传位点的几个特定等位基因重组为一组有限的单一菌株背景上的基因型。频繁、方便的访问对于调查人员来说至关重要，以便它们可以进行交配，从连续的窝中收集组织活检以确定基因型，并且观察动物的身体和行为特征（表型），特别是它们的倾向形成肿瘤。此外，许多 AECC 调查人员使用特定的无病原体啮齿动物，这些动物要么具有发生自发性肿瘤的遗传倾向或免疫缺陷且耐受肿瘤异种移植物作为研究特定过程（例如血管生成和转移）以及测试抗肿瘤药物的模型疗法。这些研究人员必须经常接触他们的动物以植入肿瘤组织、监测肿瘤生长、进行各种治疗并收集组织活检。畜牧业服务、兽医护理以及全面的啮齿动物质量保证和检疫计划由动物研究所 (IAS) 提供给 AECC。 IAS由三个教员实验室组成动物兽医（包括主任）、兽医技术员三名、工作人员约 40 名动物管理员、一名设施经理、两名畜牧主管以及办公室行政人员。除了提供兽医护理外，IAS 兽医还提供动物护理和动物护理的监督使用计划，监控法规遵从性，并为以下人员举办小组和个人培训课程调查人员。病理诊断服务由兽医病理学家提供，他还指导组织病理学共享资源。