Interactions Between Agrobacterium and Host Plants

农杆菌与宿主植物之间的相互作用

基本信息

批准号：
7877873
负责人：
Stephen C. Winans
金额：
$ 46.37万
依托单位：
CORNELL UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1989
资助国家：
美国
起止时间：
1989-07-01 至 2012-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7877873
关键词：
Aeromonas Agrobacterium Animals Antibiotics Apoproteins Bacteria Binding Biochemical Bioinformatics Biology Bioluminescence Burkholderia Cell Communication Cells Chemicals Chromosomes Collaborations Communication Complex DNA-Directed RNA Polymerase Development Diffuse Ecology Enterococcus faecalis Erwinia Family Family member Fruit Functional RNA Gene Expression Regulation Gene Targeting Genes Genetic Goals Health Homologous Gene Human In Vitro Laboratories Leadership Life Ligands Mediating Microbe Microbial Biofilms Modeling Molecular Conformation Myxococcus xanthus Nutrient Pheromone Pheromone Receptors Physiological Plant Tumor-Inducing Plasmids Plants Plasmids Play Population Density Positioning Attribute Production Property Protein Binding Proteins Proteobacteria Proteolysis Pseudomonas Pseudomonas aeruginosa RNA Ralstonia Reporting Research Personnel Resistance Rhizobium radiobacter Ribosomes Role Signal Transduction Small RNA Sorting - Cell Movement Streptomyces Structure System Testing Vibrio Vibrio fischeri Virulence Virulence Factors Yersinia Yersinia enterocolitica Yersinia pestis base cell envelope density fascinate homoserine lactone luxR protein mutant octopine pathogen promoter quorum sensing receptor reconstitution response transcription factor tumor

项目摘要

DESCRIPTION (provided by applicant): The goal of this laboratory is continue to the develop the Agrobacterium-plant pathosystem as a leading model for host-microbe interactions and for pheromone-mediated bacterial communication. In the past 15 years, cell-cell communication via diffusible chemical signals has come to be appreciated as an important property of many bacteria, especially those associated with plant, animal, or human hosts. Among these signals, acyl-homoserine lactones (AHLs) are especially widespread among the proteobacteria. AHLs are generally synthesized by proteins resembling the Luxl protein of Vibrio fischeri, and detected by transcription factors resembling the V. ficsheri LuxR protein. My lab focuses on genetic and biochemical studies of several LuxR-type proteins. One of these systems, Tral and TraR of Agrobacterium tumefaciens regulate genes required for the conjugation and vegetative replication of a large tumor-inducing plasmid. Cepl and CepR of Burkholderia cenocepacia regulate many genes on several chromosomes and plasmid of this bacterium, while Yenl and YenR of Yersinia enterocolitica regulate an unknown number of genes, including yenS, which appears to encode a small regulatory RNA. We will continue to test the hypothesis that TraR and CepR require AHL for correct folding, and must bind this pheromone during their own synthesis on ribosomes. We will also study how the activating region or regions of TraR interact with RNA polymerase. We will identify target genes activated by CepR and YenR. YenR is unusual in that it is active as an apo-protein and its activity is inhibited by AHLs. We will try to reconstitute this inhibition in vitro. Finally, we will test the idea that yenS encodes a small non-coding RNA and determine whether this sRNA regulates the stability of target mRNAs by forming heteroduplexes. Direct targets of YenR and YenS will be identified and assessed for their roles in the biology and ecology of Y. enterocolitica and Y. pestis.

描述（由申请人提供）：该实验室的目的是继续开发农业植物的病理系统，作为宿主 - 微生物相互作用和信息素介导的细菌交流的领先模型。在过去的15年中，通过可扩散的化学信号进行的细胞 - 细胞通信已被视为许多细菌的重要特性，尤其是与植物，动物或人类宿主相关的细菌的重要特性。在这些信号中，酰基 - 耶洛梅林内酯（AHL）在蛋白质细菌中尤其普遍。通常，AHL是通过类似于Fibrio Fischeri的Luxl蛋白的蛋白质来合成的，并通过类似于V. ficsheri luxr蛋白的转录因子检测到。我的实验室专注于几种luxR型蛋白的遗传和生化研究。这些系统之一，tumefaciens的tral和Trar调节了大型肿瘤诱导质粒所需的基因。 Burkholderia cenocepacia的CEPL和CEPR调节该细菌的几个染色体和质粒上的许多基因，而小肠结肠炎的Yenl和Yenr则调节了不明数的基因，其中包括Yens，Yens似乎编码了一个小的调节性RNA。我们将继续检验以下假设：Trar和CEPR需要AHL才能正确折叠，并且必须在其自身对核糖体合成过程中结合此信息素。我们还将研究TRAR的激活区域或区域如何与RNA聚合酶相互作用。我们将确定由CEPR和Yenr激活的靶基因。 Yenr是不寻常的，因为它作为Apo蛋白具有活性，并且其活性受到AHL的抑制。我们将尝试在体外重新建立这种抑制作用。最后，我们将测试Yens编码一个小的非编码RNA的想法，并确定该SRNA是否通过形成异源化合体来调节目标mRNA的稳定性。 Yenr和Yens的直接靶标将被确定并评估其在Y. entocolitica和Y. Pestis的生物学和生态学中的作用。