Ultraflex II Mass Spectrometer for Biomedical Research

用于生物医学研究的 Ultraflex II 质谱仪

• 批准号: 7214985
• 负责人: CLIVE A SLAUGHTER
• 金额: $ 44.95万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214985
• 关键词: Arts; Attention; Bioinformatics; Biomedical Research; Biotechnology; Cell membrane; Complex; Core Facility; Development; Digestion; Disease; Drug Delivery Systems; Evolution; Funding; Future; Genetic; Health; Histocompatibility Testing; Image; Institution; Lipids; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Methodology; Molecular; Pathologic; Pattern; Pharmacologic Substance; Pharmacology; Post-Translational Protein Processing; Procedures; Proteins; Proteome; Proteomics; Purpose; Range; Research; Saint Jude Children' s Research Hospital; Sampling; Science; Techniques; Technology; Tissue Differentiation; Tissue Sample; Tissues; Tumor Cell Biology; United States National Institutes of Health; cancer therapy; cell type; developmental neurobiology; drug distribution; instrument; instrumentation; mass spectrometer; programs; protein expression

DESCRIPTION (provided by applicant): The new science of proteomics, which seeks to provide global descriptions of protein expression and post-translational modification patterns in normal and pathologic tissues, is founded on the extraordinary sensitivity and discriminating power of mass spectrometry for the study of complex assemblages of proteins. The methodology for mass spectrometric analysis of entire proteomes is undergoing rapid evolution. In particular, new instrumentation and procedures are emerging that circumvent the need to employ proteolytic digestion to break proteins into small fragments for the purpose of rendering them tractable to mass spectrometric analysis. The new techniques open significant opportunities for sampling components of the proteome that have hitherto received little attention, and for investigating details of tissue differentiation and function in health and disease. To take advantage of these developments, the Hartwell Center for Bioinformatics and Biotechnology, a core facility at St. Jude Children's Research Hospital, is seeking to expand its mass spectrometry capabilities. A state-of-the-art instrument that uses matrix-assisted laser desorption/ionization time- of-flight mass spectrometry will be deployed for multidimensional analysis of protein expression in undigested tissue samples, and for direct imaging of tissue sections to study patterns of tissue differentiation. The same instrumentation will enable imaging studies of lipid distribution and drug targeting previously unavailable at the institution. We have identified six major, NIH funded research programs under way at St. Jude Children's Research Hospital with immediate need for this technology. These programs cover a broad range of topics including Pharmaceutical Science, Molecular Pharmacology, Genetics and Tumor Cell Biology, and Developmental Neurobiology. There are many other programs that have similar needs, or will have in the near future. The instrument will be incorporated into the Hartwell Center for present and future needs in basic biomedical research. Relevance St. Jude Children's Research Hospital in Memphis is seeking to deploy a mass spectrometer with new capabilities for tissue imaging to study normal and pathologic tissue types. The instrument will provide information about the proteins responsible for functional specialization of different cell types, the distribution of the various lipids that form cell membranes, and the distribution of drugs that are administered for the treatment of cancers.

描述（由申请人提供）：蛋白质组学的新科学旨在提供正常和病理组织中蛋白质表达和翻译后修饰模式的整体描述，其基础是质谱法对研究蛋白质的非凡灵敏度和辨别能力。蛋白质的复杂组合。整个蛋白质组的质谱分析方法正在快速发展。特别是，新的仪器和程序正在出现，避免了使用蛋白水解消化将蛋白质分解成小片段的需要，从而使它们易于进行质谱分析。这些新技术为迄今为止很少受到关注的蛋白质组成分取样以及研究健康和疾病中组织分化和功能的细节提供了重要的机会。为了利用这些发展，圣裘德儿童研究医院的核心设施哈特韦尔生物信息学和生物技术中心正在寻求扩大其质谱能力。将部署使用基质辅助激光解吸/电离飞行时间质谱的最先进仪器，对未消化组织样本中的蛋白质表达进行多维分析，并对组织切片进行直接成像以研究组织分化。相同的仪器将使该机构以前无法进行的脂质分布和药物靶向成像研究成为可能。我们已经确定了圣裘德儿童研究医院正在进行的 6 个由 NIH 资助的主要研究项目，这些项目迫切需要这项技术。这些课程涵盖广泛的主题，包括药物科学、分子药理学、遗传学和肿瘤细胞生物学以及发育神经生物学。还有许多其他程序有类似的需求，或者在不久的将来会有类似的需求。该仪器将并入哈特韦尔中心，以满足基础生物医学研究当前和未来的需求。相关性 孟菲斯的圣裘德儿童研究医院正在寻求部署具有组织成像新功能的质谱仪，以研究正常和病理组织类型。该仪器将提供有关不同细胞类型功能特化的蛋白质、形成细胞膜的各种脂质的分布以及用于治疗癌症的药物分布的信息。