TRANSPOSABLE ELEMENT DERIVED NON-CODING RNA REGULATORY SEQUENCES IN THE HUMAN G

人类 G 中转座元件衍生的非编码 RNA 调控序列

• 批准号: 7723210
• 负责人: I. KING JORDAN
• 金额: $ 0.05万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723210
• 关键词: Bioinformatics; Class; Computer Retrieval of Information on Scientific Projects Database; DNA Transposable Elements; Facility Construction Funding Category; Functional RNA; Funding; Gene Expression; Gene Targeting; Genes; Genome; Genomics; Grant; Human; Institution; Location; Maps; Matrix Attachment Regions; MicroRNAs; Pattern; Positioning Attribute; Regulatory Element; Research; Research Personnel; Resolution; Resources; Ribonucleic Acid Regulatory Sequences; Role; Small Interfering RNA; Source; Students; Supercomputing; Techniques; United States National Institutes of Health; scaffold

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The evolutionary origins and functional roles of human non-coding sequences, which make up the vast majority (~98.5%) of the genome, remain largely unknown. Many of these sequences are derived from transposable elements (TEs), and the aim of my research is to explore the contribution of TEs to regulatory sequences that control the expression of human genes. Our previous studies have shown that the remnants of numerous TE insertions have been coopted to provide several distinct classes of human regulatory sequences including cis-regulatory elements and scaffold/matrix attachment regions. Together with graduate student Jittima Piriyapongsa, I propose to further explore the relationship between TEs and human regulatory sequences with an emphasis on non-coding RNA regulatory sequences such as small interfering RNAs (siRNAs) and micro RNAs (miRNAs). We plan to use the Bioinformatics Resource at the Pittsburgh Supercomputing Center to aid in the construction high resolution genomic maps that combine the locations of human non-coding RNA regulatory sequences and their targets, characterized by recently developed high throughput experimental techniques, with TE sequence positions. These maps will then be employed in cross-species comparisons, across different levels of evolutionary relatedness, in an attempt to characterize the evolutionary dynamics of TE-derived non-coding RNA regulatory sequences. Finally, patterns of gene expression will be used in efforts to discern the particular regulatory effects exerted on target genes by TE-derived non-coding RNAs, and where possible, to explore evolutionary changes in expression wrought by lineage-specific insertions of TE regulatory sequences.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 人类非编码序列的进化起源和功能作用，构成了基因组的绝大多数（〜98.5％），这在很大程度上是未知的。这些序列中的许多序列源自可转座元素（TES），我的研究目的是探索TES对控制人类基因表达的调节序列的贡献。我们先前的研究表明，已经掌握了许多TE插入的残余物，以提供几类不同的人类调节序列，包括顺式调节元件和支架/基质/基质附着区域。我与研究生Jittima piriyapongsa一起进一步探索TES与人类调节序列之间的关系，重点是非编码RNA调节序列，例如小干扰RNA（siRNA）和微RNA（miRNA）。我们计划在匹兹堡超级计算中心使用生物信息学资源来帮助建造高分辨率基因组图，这些基因组图结合了人类非编码RNA调节序列的位置及其目标，其特征是最近开发的高吞吐量实验技术与TE TE序列位置， 。然后，这些地图将用于跨物种比较，跨不同级别的进化相关性，以试图表征TE衍生的非编码RNA调节序列的进化动力学。最后，将使用基因表达的模式用于努力，以辨别由TE衍生的非编码RNA对靶基因施加的特定调节作用，并在可能的情况下探索谱系调节序列的谱系特异性插入中表达的进化变化。