CORE--NEUROPATHOLOGY CORE

核心--神经病理学核心

• 批准号: 7628486
• 负责人: Jean Paul Vonsattel
• 金额: $ 21.1万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7628486
• 关键词: Aliquot; Alzheimer' s Disease; Apolipoprotein E; Area; Arts; Autopsy; Bar Codes; Biochemical; Blood; Brain; Brain Diseases; Classification; Clinical; Collection; Computer software; DNA; Data; Development; Diagnosis; Diagnostic; Disease; Dissection; Educational process of instructing; Ensure; Evaluation; Formalin; Freezing; Genotype; Goals; Grant; Gray unit of radiation dose; Guidelines; Harvest; Histopathology; Human; Individual; Internet; Investigation; Label; Lead; Lesion; Leukocytes; Libraries; Life; Location; Logistics; Measures; Mitochondria; Molecular Biology; Nature; Neuroanatomy; Neurology; Numbers; Paraffin Embedding; Pathologic; Pathology; Patients; Principal Investigator; Procedures; Process; Protocols documentation; Psychiatry; RNA; Range; Reporting; Research; Research Personnel; Research Project Grants; Resources; Sampling; Scientist; Side; Site; Solutions; Sorting - Cell Movement; Specific qualifier value; Specimen; Staging System; Symptoms; Technology; Time; Tissues; Training; Universities; Update; Whole Blood; brain research; brain tissue; demographics; enzyme activity; immunocytochemistry; improved; member; mild neurocognitive impairment; morphometry; neuropathology; neurosurgery; new technology; patient oriented; programs; software development; tissue preparation; white matter

The Alzheimer's Disease Research Center (ADRC) Neuropathology Core has been completely reorganized during the present grant period. It provides state-of-the-art neuropathological examination of brains obtained from ADRC autopsy cases. Brains are processed in a standardized fashion, with detailed histopathological assessment, by means of examination of a standardized set of brain sections, now illustrated on our website. Standardized reports with extensive data are provided, including histopathological diagnoses using accepted neuropathological criteria and CERAD, NLA-Reagan, and Braak & Braak staging systems. The new procedures enhance the efficiency of the ADRC brain bank, and the value of collected brains to investigators whose specific research projects depend upon the availability of carefully prepared and examined tissue. Each ADRC brain is bisected, with one half formalin-fixed, and thoroughly examined neuropathologically. The other half is extensively dissected yielding up to 150 or more fresh frozen samples from precisely specified regions, harvested and processed at time of autopsy. Only trained neuropathologists perform the dissection to ensure consistency and accurate assessment of the tissue during the processing. The samples are categorized both diagnostically and qualitatively, electronically-tracked, ready for instant disbursement, and suitable for a wide range of investigations using various modern biochemical technologies. In addition to managing the collections of fresh frozen, and formalin-fixed brain samples, DNA and RNA samples are prepared and then stored from frozen brains, as is DNA from blood of living ADRC patients. The voluminous nature of the Neuropathology Core samples requires recording many crucial details. Thus, we have developed software that: (i) generates bar-code labels and tracks samples; (ii) pre-selects among thousand of samples those that best match the requirements of investigators according to the demographics and pathological changes; (iii) provides freezer cabinet coordinates for each sample; (iv) traces vacant freezer space after sample disbursements; and (v) registers recipient investigators. The Neuropathology Core actively supports numerous fertile interactions between neuroscientists, geneticists, clinicians and other AD researchers.

阿尔茨海默病研究中心 (ADRC) 神经病理学核心在目前的资助期内已完全重组。它提供从 ADRC 尸检病例中获得的最先进的大脑神经病理学检查。通过检查一组标准化的大脑切片，以标准化的方式处理大脑，并进行详细的组织病理学评估，现在在我们的网站上进行了说明。提供包含大量数据的标准化报告，包括使用公认的神经病理学标准和 CERAD、NLA-Reagan 和 Braak & Braak 分期系统进行的组织病理学诊断。新程序提高了 ADRC 脑库的效率，以及收集的大脑对于研究人员的价值，这些研究人员的具体研究项目取决于精心准备和检查的组织的可用性。每个 ADRC 大脑都被一分为二，一半用福尔马林固定，并进行彻底的神经病理学检查。另一半经过广泛解剖，从精确指定的区域产生多达 150 个或更多新鲜冷冻样本，并在尸检时收获和处理。只有训练有素的神经病理学家才能执行 解剖以确保处理过程中组织的一致性和准确评估。这些样本经过诊断和定性分类，进行电子跟踪，可供即时支付，适合使用各种现代生化技术进行广泛的研究。除了管理新鲜冷冻和福尔马林固定脑样本的收集外，还从冷冻脑中制备 DNA 和 RNA 样本，然后保存，就像从活体 ADRC 患者血液中提取 DNA 一样。神经病理学核心样本的大量性质需要记录许多关键细节。因此，我们开发了以下软件：(i) 生成条形码标签并跟踪样本； (ii) 根据人口统计和病理变化，从数千个样本中预先选择最符合研究者要求的样本； (iii) 提供每个样品的冷冻柜坐标； (iv) 追踪样品支出后冷冻室的空置空间； (v) 登记接收调查员。神经病理学核心积极支持神经科学家、遗传学家、临床医生和其他 AD 研究人员之间的大量互动。