REACTIVE OXYGEN AND NITROGEN SPECIES IN RAW 2647 MACROPHAGE CELLS

原始 2647 巨噬细胞中的活性氧和氮物种

• 批准号: 7721396
• 负责人: THOMAS Comey SQUIER
• 金额: $ 2.4万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-08 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721396
• 关键词: Aging; Cell physiology; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Disease; Funding; Goals; Grant; Immune response; Infection; Inflammation; Inflammatory; Institution; Mass Spectrum Analysis; Modification; Nitrogen; Oxygen; Pathology; Pathway interactions; Performance; Process; Proteins; Proteome; Reactive Oxygen Species; Research; Research Personnel; Resolution; Resources; Source; Therapeutic; Therapeutic Intervention; United States National Institutes of Health; base; macrophage; repaired

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Reactive oxygen species (ROS) are required for mounting an effective immune response, yet ROS also produce detrimental oxidative modifications to host proteins. Previously we demonstrated that ROS induce oxidative modifications to host proteins and induce repair processes (1). Thus, the identification of changes in protein abundance as well as modifications during inflammation (e.g. aging or infection), will further our understanding of how ROS contribute to disease processes. In addition, characterization of changes in protein complexes will aid in the identification of vital pathways for targeted therapeutic intervention. Our ultimate goals are to identify changes in cellular process and the proteome based upon inflammation, determine oxidative modifications to proteins due to ROS, and determine alterations in protein complexes using high resolution separations and high performance mass spectrometry. These findings will be instrumental in defining the underlying cause of inflammatory disease and associated pathologies with the end goal of development of therapeutic interventions (e.g. pharmacological therapeutics) to curb disease processes.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 活性氧 (ROS) 是有效免疫反应所必需的，但 ROS 也会对宿主蛋白产生有害的氧化修饰。 之前我们证明 ROS 会诱导宿主蛋白的氧化修饰并诱导修复过程 (1)。 因此，识别蛋白质丰度的变化以及炎症（例如衰老或感染）期间的修饰，将进一步了解 ROS 如何促进疾病过程。 此外，蛋白质复合物变化的表征将有助于识别靶向治疗干预的重要途径。 我们的最终目标是识别基于炎症的细胞过程和蛋白质组的变化，确定由于 ROS 引起的蛋白质氧化修饰，并使用高分辨率分离和高性能质谱确定蛋白质复合物的变化。 这些发现将有助于确定炎症性疾病和相关病理的根本原因，最终目标是开发治疗干预措施（例如药物疗法）来遏制疾病进程。