IDENT OF POST-TRANS MOD & PROTEIN COMPLEXES UNDER CONDITIONS OF OXIDATIVE STRES

后传输 MOD 的识别

• 批准号: 7359110
• 负责人: THOMAS Comey SQUIER
• 金额: $ 6.3万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359110
• 关键词: IDENT; POST; TRANS; MOD; PROTEIN

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our goal is to understand the mechanisms underlying observed alterations in calcium homeostasis and the oxidation and nitration of intracellular proteins that occur under a range of biological pathologies, including biological aging. In this work we will focus on stress-response signaling pathways in mouse macrophages involving the critical calcium regulatory protein calmodulin (CaM). The mass spectrometry tools available to us will be used to 1) identify CaM binding partners and their involvement in signaling pathways 2) investigate the role of nitrated tyrosine in the regulatory processes, using CaM as a model protein and 3) explore the use of CaM as a biosensor to differentiate between types of oxidative stress.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。我们的目标是了解在一系列生物病理学（包括生物衰老）下发生的钙稳态变化以及细胞内蛋白质氧化和硝化的潜在机制。在这项工作中，我们将重点关注小鼠巨噬细胞中涉及关键钙调节蛋白钙调蛋白（CaM）的应激反应信号通路。我们可用的质谱工具将用于 1) 识别 CaM 结合伴侣及其在信号传导通路中的参与 2) 使用 CaM 作为模型蛋白研究硝化酪氨酸在调节过程中的作用 3) 探索 CaM 的用途作为区分氧化应激类型的生物传感器。