HOST INTERACTIONS OF THE YEAST RETROTRANSPOSON TY3

酵母逆转录转座子 TY3 的宿主相互作用

• 批准号: 7723767
• 负责人: SUZANNE SANDMEYER
• 金额: $ 0.73万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723767
• 关键词: Affect; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Genomic Library; Grant; Institution; Mediating; Mutation; Nucleosome Core Particle; Open Reading Frames; Proteins; Research; Research Personnel; Resources; Retrotransposon; Retroviridae; Scanning; Source; Structural Protein; Testing; United States National Institutes of Health; Virus-like particle; Yeasts; mutant; particle; trafficking; vector; yeast two hybrid system

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ty3 produces virus-like particles similar to the core particles of retroviruses. In the case of Ty3 however, about 60 Ala scanning mutations in the structural protein open reading frame are available with which to study interactions of the structural protein with itself and with host proteins. We have constructed Gag3 insertions in two hybrid bait and activation vectors which target Gal4-mediated control. The structural protein (Gag3) has been shown to interact with itself but to be affected in certain mutants. Two of the mutations are candidates for affecting interactions between Gag3 and host proteins. Preliminary data suggests that these proteins might be involved in vesicular trafficking and P-body localization. We propose to screen a yeast genomic library in order to test for host proteins that interact with Ty3 Gag3 and are involved in particle assembly.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Ty3 产生类似于逆转录病毒核心颗粒的病毒样颗粒。 然而，就 Ty3 而言，结构蛋白开放阅读框中约有 60 个 Ala 扫描突变，可用于研究结构蛋白与其自身以及与宿主蛋白的相互作用。 我们在两个混合诱饵和激活载体中构建了 Gag3 插入片段，以 Gal4 介导的控制为目标。 结构蛋白 (Gag3) 已被证明可以与其自身相互作用，但在某些突变体中会受到影响。 其中两个突变是影响 Gag3 和宿主蛋白之间相互作用的候选突变。初步数据表明这些蛋白质可能参与囊泡运输和 P 体定位。我们建议筛选酵母基因组文库，以测试与 Ty3 Gag3 相互作用并参与颗粒组装的宿主蛋白。