High Throughput DNA Sequencer

高通量 DNA 测序仪

• 批准号: 7594911
• 负责人: SUZANNE SANDMEYER
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594911
• 关键词: Administrator; Area; Bacterial Genome; Biomedical Research; Code; Commit; Core Facility; DNA; DNA Resequencing; DNA Sequence; Data; Disease; Disease susceptibility; Epigenetic Process; Equipment; Experimental Designs; Funding; Gene Expression; Genetic Transcription; Genetic Variation; Genome; Genomics; Human; Human Genome; Knowledge; Malignant Neoplasms; Malignant neoplasm of lung; Modeling; Operative Surgical Procedures; Protein Binding; Protein Microchips; Proteins; RNA Splicing; Regulation; Research; Research Personnel; Research Training; Sheep; Site; System; Technology; Time; Untranslated RNA; Variant; Virulence; genetic regulatory protein; genome sequencing; genome wide association study; genome-wide; genome-wide analysis; instrument; public health relevance; tool

DESCRIPTION (provided by applicant): We propose to purchase a Solexa Genome Analyzer System from Illumina. High throughput sequence data will positively impact campus research. Twenty-one investigators will be enabled to perform projects in the areas of: 1) genome resequencing to identify bacterial virulence determinants and to correlate human gene variants with disease susceptibility; 2) interaction of proteins with chromosomal DNA throughout the genome in order to understand transcriptional and epigenetic regulation; and 3) genomewide gene expression to enable understanding of the comprehensive picture of transcription from coding sequences to noncoding RNAs. High throughput DNA sequence technologies have two very significant advantages over current technologies. First, data are collected so efficiently that fundamental changes in experimental design are enabled, for example by allowing rapid resequencing of bacterial genomes for variations association with virulence or resequencing segment of the human genome for SNP association studies. Second, most applications of sequencing are less dependent on what is already known than microarrays, the other major genome wide analysis tool which is completely dependent upon knowledge of target genome sequence or in the case of some gene expression arrays, also dependent on knowledge about what is transcribed and even what is spliced. The sequencer will be added to the current DNA and Protein MicroArray Facility to form a UCI Genomics Core Facility. Funds have been committed by the campus to complete purchase of the Solexa instrument and extended warranties and purchase the necessary accessory equipment, and to hire a full time technician and part time systems administrator to staff this expansion. Acquisition of the sequencer will have a high positive impact on research and training at this rapidly growing UC campus. PUBLIC HEALTH RELEVANCE: A consortium of investigators from across campus proposes to acquire a Solexa high throughput sequencer from Illumina for the UCI Genomics Core Facility. High throughput sequencing dramatically increases the amount of sequence which can be collected in one experimental operation. Addition of this instrument to the Core Facility will enable exciting biomedical research. Projects include sequencing entire bacterial genomes to discover virulence determinants, analysis of human genomes to identify sequence variants associated with disease, identification of sites across the human genome where cancer-related regulatory proteins bind to control gene expression, and quantification of changes in gene expression in a sheep model for lung cancer.

描述（由申请人提供）：我们建议从Illumina购买Solexa基因组分析仪系统。高通量序列数据将对校园研究产生积极影响。将使21个研究人员能够在以下方面执行项目：1）基因组重新定价以鉴定细菌毒力决定因素并将人类基因变异与疾病易感性相关联； 2）蛋白质与整个基因组中染色体DNA的相互作用，以了解转录和表观遗传学调节； 3）全基因组基因表达，以了解从编码序列到非编码RNA的转录的全面图片。高吞吐量DNA序列技术比当前技术具有两个非常重要的优势。首先，可以有效地收集数据，以至于实现了实验设计的基本变化，例如，通过允许将细菌基因组快速重新取代，以使其与毒力变化或人类基因组进行SNP关联研究的变异或重新配置段。其次，测序的大多数应用都比微阵列（完全取决于目标基因组序列的知识或某些基因表达阵列的知识，也取决于对知识的知识，它都依赖于微阵列，而不是微阵列。被转录，甚至是剪接的。测序仪将被添加到当前的DNA和蛋白质微阵列设施中，以形成UCI基因组核心设施。校园已投入资金，以完成购买Solexa仪器和扩展保修并购买必要的配件设备，并聘请全职技术人员和兼职系统管理员来人员来工作。在这个迅速增长的UC校园中，对音序器的获取将对研究和培训产生高积极影响。 公共卫生相关性：来自校园各地的一个调查人员联盟提议从Illumina获得UCI基因组学核心设施的Solexa高吞吐量测序仪。高通量测序大大增加了可以在一个实验操作中收集的序列的量。在核心设施中增加该工具将使令人兴奋的生物医学研究。项目包括对整个细菌基因组进行测序以发现毒力决定因素，分析人类基因组以鉴定与疾病相关的序列变异，鉴定与癌症相关的调节蛋白与对照基因表达结合的人类基因组的鉴定，以及对基因表达的量化的定量肺癌的绵羊模型。