ANALYSIS OF THE MICROTUBULE-ASSOCIATED PROTEOME DURING NEURONAL MORPHOGENESIS

神经元形态发生过程中微管相关蛋白质组的分析

• 批准号: 7723688
• 负责人: Shelley L Halpain
• 金额: $ 0.08万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7723688
• 关键词: Axon; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; Cytoskeleton; Dendrites; Development; Funding; Grant; Institution; Microtubule-Associated Proteins; Microtubules; Morphogenesis; Neurites; Neurons; Play; Process; Proteome; Proteomics; Research; Research Personnel; Resources; Role; Source; United States National Institutes of Health; base

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During nerve cell development, postmitotic neurons transform from seemingly unpolarized cells into highly polarized ones. These highly polarized neurons contain long cellular processes termed neurites that are filled with the microtubule (MT) cytoskeleton, and will later mature into axons or dendrites. It has been shown that the morphological change during neuronal development depends on the organization of the MT cytoskeleton. Although many of MT-associated proteins are known to play important roles during neuronal morphogenesis, a comprehensive proteomic analysis focusing on the MT cytoskeleton during this process is lacking. To better understand the mechanisms controlling neurite formation, we propose to perform MS-based proteomic analysis on MT-associated proteins before and during this process.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 在神经细胞发育过程中，有丝分裂后神经元从看似非极化细胞转变为高度极化的细胞。这些高度极化的神经元包含长细胞过程，称为填充微管（MT）细胞骨架的神经突，后来将成熟成轴突或树突。已经表明，神经元发育过程中的形态变化取决于MT细胞骨架的组织。尽管已知许多与MT相关的蛋白在神经元形态发生过程中起着重要作用，但是在此过程中，围绕MT细胞骨架的全面蛋白质组学分析缺乏。为了更好地了解控制神经突的机制，我们建议在此过程之前和期间对MT相关蛋白进行基于MS的蛋白质组学分析。