CRYSTAL STRUCTURE OF THE CED-9/CED-4/CED-3 TERNARY COMPLEX

CED-9/CED-4/CED-3 三元络合物的晶体结构

• 批准号: 7726242
• 负责人: YIGONG SHI
• 金额: $ 5.01万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-18 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7726242
• 关键词: Apoptosis; Caenorhabditis elegans; Caspase; Cell Death; Complex; Computer Retrieval of Information on Scientific Projects Database; Development; Funding; Genetic; Grant; Homeostasis; Institution; Molecular Structure; Play; Proteins; Research; Research Personnel; Resources; Role; Source; Structure; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Apoptosis plays an essential role in the development and homeostasis of metazoans. The genetic characterization of programmed cell death (apoptosis) in C. elegans identified four proteins, CED-3, CED-4, CED-9, and EGL-1, that collectively control the onset of apoptosis. CED-3 is a caspase and its activation depends on CED-4. CED-9 antagonize the function of CED-4 through an unknown mechanism. CED-3, CED-4, and CED-9 form a hetero-oligomer. To elucidate the mechanisms of cell death control in C. elegans, we crystallized the ternary complex of CED-3/CED-4/CED-9. We plan to determine the structure by molecular replacement or by MAD.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 凋亡在后生动物的发展和稳态中起着至关重要的作用。秀丽隐杆线虫中程序性细胞死亡（凋亡）的遗传表征鉴定出四种蛋白质CED-3，CED-4，CED-9和EGL-1，这些蛋白共同控制了凋亡的开始。 CED-3是caspase，其激活取决于CED-4。 CED-9通过未知机制拮抗Ced-4的功能。 CED-3，CED-4和CED-9形成异质 - 寡聚物。为了阐明秀丽隐杆线虫中细胞死亡控制的机制，我们结晶了CED-3/CED-4/CED-9的三元复合物。我们计划通过分子置换或疯狂确定结构。