ANIONIC LIPIDS ENRICHED AT THE EXPORTAL OF STREPTOCOCCUS PYOGENES

化脓性链球菌出口处富含阴离子脂质

• 批准号: 7721561
• 负责人: Jason W. Rosch
• 金额: $ 0.27万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721561
• 关键词: Acridine Orange; Active Sites; Behavior; Cardiolipins; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Lipids; Location; Membrane; Membrane Microdomains; Phosphatidyl glycerol; Phosphatidylglycerols; Phospholipids; Play; Protein Secretion; Research; Research Personnel; Resources; Role; Source; Staining method; Stains; Streptococcus pyogenes; United States National Institutes of Health; intracellular protein transport; mutant; protein folding; protein transport

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ExPortal of Streptococcus pyogenes is a membrane microdomain dedicated to the secretion and folding of proteins. We investigated the lipid composition of the ExPortal by examining the distribution of anionic membrane phospholipids. Staining with 10-N-nonyl-acridine orange revealed a single microdomain enriched with an anionic phospholipid whose staining characteristics and behavior in a cardiolipin-deficient mutant were characteristic of phosphatidylglycerol. Furthermore, the location of the microdomain corresponded to the site of active protein secretion at the ExPortal. These results indicate that the ExPortal is an asymmetric lipid microdomain, whose enriched content of anionic phospholipids may play an important role in ExPortal organization and protein trafficking.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 化脓性链球菌的 ExPortal 是一个专门负责蛋白质分泌和折叠的膜微结构域。我们通过检查阴离子膜磷脂的分布来研究 ExPortal 的脂质组成。用 10-N-壬基-吖啶橙染色显示出富含阴离子磷脂的单个微域，其在心磷脂缺陷突变体中的染色特征和行为是磷脂酰甘油的特征。此外，微结构域的位置对应于 ExPortal 活性蛋白分泌的位点。这些结果表明ExPortal是一个不对称的脂质微结构域，其丰富的阴离子磷脂含量可能在ExPortal组织和蛋白质运输中发挥重要作用。