ANIONIC LIPIDS ENRICHED AT THE EXPORTAL OF STREPTOCOCCUS PYOGENES

化脓性链球菌出口处富含阴离子脂质

• 批准号: 7721561
• 负责人: Jason W. Rosch
• 金额: $ 0.27万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721561
• 关键词: Acridine Orange; Active Sites; Behavior; Cardiolipins; Characteristics; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Lipids; Location; Membrane; Membrane Microdomains; Phosphatidyl glycerol; Phosphatidylglycerols; Phospholipids; Play; Protein Secretion; Research; Research Personnel; Resources; Role; Source; Staining method; Stains; Streptococcus pyogenes; United States National Institutes of Health; intracellular protein transport; mutant; protein folding; protein transport

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ExPortal of Streptococcus pyogenes is a membrane microdomain dedicated to the secretion and folding of proteins. We investigated the lipid composition of the ExPortal by examining the distribution of anionic membrane phospholipids. Staining with 10-N-nonyl-acridine orange revealed a single microdomain enriched with an anionic phospholipid whose staining characteristics and behavior in a cardiolipin-deficient mutant were characteristic of phosphatidylglycerol. Furthermore, the location of the microdomain corresponded to the site of active protein secretion at the ExPortal. These results indicate that the ExPortal is an asymmetric lipid microdomain, whose enriched content of anionic phospholipids may play an important role in ExPortal organization and protein trafficking.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 链球菌为链球菌的出口是一种用于蛋白质分泌和折叠的膜微域。我们通过检查阴离子膜磷脂的分布来研究出口的脂质组成。用10-N-氯氨基橙色染色显示出一个富含阴离子磷脂的单个微域，其磷脂缺陷型突变体中的染色特征和行为是磷脂酰甘油的特征。此外，微域的位置与出口处的活性蛋白质分泌位点相对应。这些结果表明，出口是一种不对称的脂质微域，其富集的阴离子磷脂含量可能在出口组织和蛋白质运输中起重要作用。