NEW TECHNOLOGIES FOR TIME-RESOLVED INVESTIGATIONS

用于时间分辨调查的新技术

• 批准号: 7721723
• 负责人: Tong Lu
• 金额: $ 4.45万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721723
• 关键词: Computer Retrieval of Information on Scientific Projects Database; Cryoelectron Microscopy; Deposition; Devices; Freezing; Funding; Goals; Grant; Institution; Investigation; Microfluidics; Research; Research Personnel; Resolution; Resources; Source; Specimen; Technology; Testing; Time; United States National Institutes of Health; Work; abstracting; base; design; macromolecule; millisecond; new technology; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: Currently, microfluidics technology is being applied to develop micromixers for time resolved cryo-electron microscopy (TRCEM) application. TRCEM requires fast and homogeneous premixing of tiny amounts of macromolecules in the time scale of milliseconds or less. Work is also just beginning in which this technology will be applied to the problems of specimen deposition onto EM grids and rapid freezing. Our goal for the remainder of this grant is to design and test a microfluidics-based device that allows TRCEM experiments to be conducted routinely with millisecond time resolution and requires only the small amounts of biomolecules that are often available to researchers.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 抽象的： 目前，微流体技术正被应用于开发用于时间分辨冷冻电子显微镜（TRCEM）应用的微混合器。 TRCEM 需要在毫秒或更短时间内快速均匀地预混合微量大分子。 这项技术将应用于解决样品沉积到电磁网格上和快速冷冻的问题，这一工作也刚刚开始。 我们剩余资金的目标是设计和测试一种基于微流体的设备，该设备允许以毫秒时间分辨率常规进行 TRCEM 实验，并且只需要研究人员通常可以获得的少量生物分子。