MOLECULAR BIOLOGY AND BIOCHEMISTRY SUPPORT CORE

分子生物学和生物化学支持核心

• 批准号: 7720922
• 负责人: ROMAN R. GANTA
• 金额: $ 70.12万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720922
• 关键词: Academic Medical Centers; Biochemistry; Bioinformatics; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Funding; Gene Chips; Gene Expression; Genes; Grant; Individual; Institution; Kansas; Mentors; Molecular Biology; Nucleotides; Outsourcing; Pattern; Polymerase Chain Reaction; Preparation; Process; Processed Genes; Proteins; RNA; Research; Research Personnel; Resources; Sampling; Services; Source; Technology; Time; Training; Transcript; United States National Institutes of Health; Universities; Validation; cell type

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Core C, the Molecular Biology and Biochemistry Support facility, provides the interface, expertise, training and mentoring in gene array analysis necessary to advance COBRE projects. Gene array technology provides a highly efficient means to screen individual cell types for gene expression patterns and changes. Gene microarray processing and bioinformatics services are available through the auspices of a K-INBRE NIH grant, thereby allowing us to focus Core support on sample preparation (analyses of quality and quantity), funding of gene microarray processing by other facilities at KSU and KUMC and of post-gene microarray validation (qRT-PCR). The Core provides gene array support by outsourcing to other facilities (at Kansas State University and Kansas University Medical Center) investigator RNA samples for gene chip preparation and hybridization; in addition, bioinformatics support to process the gene array data is provided by the COBRE Core Manager and by the service referenced above. The Core provides support through: 1) three, real-time polymerase chain reaction (PCR) thermocyclers for quantitative assessment of gene transcript expression, 2) an Agilent BioAnalyzer for determining the quality of RNA samples obtained for gene array analysis, 3) a Nanodrop spectrophotometer and spectrofluorometer for determining the quantity of nucleotide and protein samples, and 4) a DNA sequencer for verifying PCR product identity. The real-time PCR is needed to verify gene array results or analyze specific gene expression changes in more detail than is possible with the gene array.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 Core C 是分子生物学和生物化学支持设施，提供推进 COBRE 项目所需的基因阵列分析接口、专业知识、培训和指导。基因阵列技术提供了一种高效的方法来筛选单个细胞类型的基因表达模式和变化。基因微阵列处理和生物信息学服务可通过 K-INBRE NIH 资助获得，从而使我们能够将核心支持重点放在样品制备（质量和数量分析）、KSU 和 KUMC 的其他设施为基因微阵列处理提供资金以及基因后微阵列验证（qRT-PCR）。核心通过外包给其他设施（堪萨斯州立大学和堪萨斯大学医学中心）研究者 RNA 样本进行基因芯片制备和杂交来提供基因阵列支持；此外，COBRE 核心管理器和上述服务还提供处理基因阵列数据的生物信息学支持。 Core 通过以下方式提供支持：1) 三个实时聚合酶链式反应 (PCR) 热循环仪，用于定量评估基因转录本表达，2) 一个安捷伦生物分析仪，用于确定基因阵列分析所获得的 RNA 样品的质量，3) 一个 Nanodrop用于测定核苷酸和蛋白质样品数量的分光光度计和荧光分光光度计，以及 4) 用于验证 PCR 产物身份的 DNA 测序仪。需要实时 PCR 来验证基因阵列结果或比基因阵列更详细地分析特定基因表达变化。