EFFECTS OF EXERCISE TRAINING ON CARDIOVASCULAR PHYSIOLOGY

运动训练对心血管生理学的影响

• 批准号: 7725194
• 负责人: JANICE STEELE
• 金额: $ 3.16万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7725194
• 关键词: Androstenedione; Autonomic nervous system; Blood Pressure; Blood specimen; Body Composition; Brain; Cardiovascular Physiology; Catheters; Citrate (si)-Synthase; Collection; Computer Retrieval of Information on Scientific Projects Database; Coupled; Diabetes Mellitus; Effectiveness; Enzyme-Linked Immunosorbent Assay; Exercise; Female; Funding; Generations; Genes; Glutathione; Goals; Grant; Heart; Home environment; Human; Hypertension; Immersion Investigative Technique; Inbred SHR Rats; Inbred WKY Rats; Institution; Invasive; Isometric Exercise; Journals; Kidney; Liver; Maintenance; Measurement; Microarray Analysis; Nebraska; Output; Placement; Plasma; Postdoctoral Fellow; Process; Publications; Radioimmunoassay; Rattus; Relative (related person); Renal function; Research; Research Personnel; Research Project Grants; Research Subjects; Research Training; Resources; Role; Sampling; Science; Skeletal Muscle; Somatropin; Source; Streptozocin; Stress; Techniques; Tissue Sample; Tissues; Training; Training Programs; United States National Institutes of Health; Universities; Water; animal care; animal colony; animal facility; blood pressure regulation; dietary supplements; environmental stressor; grasp; novel; prevent; programs; repair enzyme; response; sedentary; thioredoxin glutathione reductase; thioredoxin reductase; urinary

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. My research training at the graduate and post-doctoral levels focused on the influence of the autonomic nervous system and renal function in the control of arterial blood pressure. I am skilled in the placement of indwelling arterial catheters for the direct measurement blood pressure and in the placement of ureteral catheters for the measurement of urinary output in rats. When I first arrived at UNK I investigated a non-invasive way to control mild hypertension (Steele, JE. Retention of relative grip effort for at-home isometric exercise. Trans. Nebraska Acad. Sci. 24: 81-88, 1997). More recently my research has focused the effectiveness of various over-the-counter nutritional supplements. I investigated the influence of androstenedione on cardiovascular function in rats and the response of the subjects to novel (water immersion) stress (Steele, JE. Influence of androstenedione on sedentary female rats. Trans. Nebraska Acad. Sci. 27: 17-24, 2001). I have also supervised several undergraduate research projects investigating the effectiveness of over-the-counter nutritional supplements in humans. Many of these projects have required the collection of blood samples and analysis of those samples by colorimetric, ELISA, or radioimmunoassay technique. One of these research projects resulted in a publication in an undergraduate research journal (Hellman, AM, and KJ Brummer. Effect of a Dietary Supplement on Body Composition and Plasma hGH Following Exercise Challenge. J. Young Investigators, Issue 7, March 2003). My long-term research goals are to continue to investigate factors which influence the control of blood pressure and the roles of stress and exercise training in blood pressure regulation. My ideal research subject is the borderline hypertensive rat (BHR), the first generation off-spring of a spontaneously hypertensive rat and a Wistar-Kyoto rats. The BHR will not spontaneously develop hypertension but is sensitive to various environmental stressors. Exercise training seems to prevent the BHR from developing hypertension even when subjected to environmental stressors. The effects of nutritional supplements in the BHR have not yet been investigated. The animal care facilities in Bruner Hall of Science on the University of Nebraska at Kearney campus have historically been quite poor, and, coupled with the recent "delayed maintenance" of the building, have prevented me from maintaining an on-going research program. The BRIN supplement has allowed us to renovate space in Bruner Hall into a modern animal facility. While this renovation is not yet complete, I have been able to maintain an animal colony and begin some preliminary projects. The first project is to examine the influence of exercise training on the efficiency of enzyme repair mechanisms in rats with streptozotocin-induced diabetes mellitus. I have collected tissue samples (heart, liver, kidney, brain, and skeletal muscle) from 50 rats and am in the process of analyzing these tissues for glutathione, glutathione reductase, thioredoxin, thioredoxin reductase, and citrate synthase (in skeletal muscle only to verify effectiveness of training program). Furthermore, I have collected tissue for gene profiling via microarray analysis.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 我在研究生和博士后水平的研究培训集中于自主神经系统和肾功能对控制动脉血压的影响。 我擅长将留置动脉导管的放置用于直接测量血压以及放置输尿管导管以测量大鼠尿量的测量。 当我第一次到达UNK时，我调查了一种控制轻度高血压的非侵入性方法（Steele，JE。保留相对抓地力的居家等静脉锻炼。Trans。NebraskaAcad。Sci。24：81-88，1997）。 最近，我的研究重点是各种非处方营养补充剂的有效性。 我研究了雄激素对大鼠心血管功能的影响以及受试者对新型（水浸）胁迫的反应（Steele，JE。雄激素对久坐的雌性大鼠的影响。 我还监督了几项研究人类非处方营养补充剂的有效性。 这些项目中的许多项目都需要收集血液样本，并通过比色，ELISA或放射免疫测定技术对这些样品进行分析。 这些研究项目之一导致了本科研究杂志（Hellman，AM和KJ Brummer。饮食补充剂对身体成分和血浆HGH的影响。 我的长期研究目标是继续研究影响血压控制以及压力和运动训练在血压调节中的作用的因素。 我理想的研究主题是边缘性高血压大鼠（BHR），这是自发性高血压大鼠的第一代偏离和wistar-kyoto大鼠。 BHR不会自发发展高血压，而对各种环境压力源敏感。 运动训练似乎也可以阻止BHR发生高血压，即使受到环境压力。 尚未研究BHR营养补充剂的影响。 内布拉斯加州大学校园内布拉斯加州布鲁纳科学院的动物护理设施历来很差，再加上最近建筑物的“延迟维护”，使我无法维持正在进行的研究计划。 Brin补充剂使我们能够将布鲁纳大厅的空间翻新成现代动物设施。 尽管这种翻新尚未完成，但我已经能够维持一个动物殖民地并开始一些初步项目。 第一个项目是检查运动训练对链霉菌素诱导的糖尿病大鼠酶修复机制效率的影响。 我已经从50只大鼠收集了组织样品（心脏，肝脏，肾脏，脑和骨骼肌），并且正在分析这些组织中的谷胱甘肽，谷胱甘肽还原酶，硫代蛋白毒素，硫氧还蛋白还原酶还原酶和柠檬酸含量合成酶（在骨骼肌肉中仅验证培训计划的有效性）。 此外，我通过微阵列分析收集了组织以进行基因分析。