SOLUTE TRANSPORT IN THE SUBCHONDRAL BONE PLATE OF OSTEOARTHRITIC JOINTS

骨关节炎关节软骨下骨板中的溶质转运

• 批准号: 7720204
• 负责人: LIYUN WANG
• 金额: $ 22.68万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7720204
• 关键词: Biological; Bone and Cartilage Funding; Bone plates; Cartilage; Communication; Computer Retrieval of Information on Scientific Projects Database; Fluorescence Recovery After Photobleaching; Foundations; Funding; Grant; In Situ; In Vitro; Institution; Interruption; Joints; Measurement; Mentors; Methods; Movement; Osteoarthrosis Deformans; Pathway interactions; Patients; Research; Research Personnel; Resources; Source; Time; United States National Institutes of Health; articular cartilage; base; bone; bone cell; in vivo; mathematical model; research study; solute

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent experiments demonstrate close interaction between cartilage and bone compartment in vitro and in vivo, where bone cells from OA patients can cause matrix breakdown of articular cartilage and interruption of contact with subchondral bone results in cartilage degeneration. However, the communication pathway that allows cartilage and bone to interact is not clear. Dr. Wang has recently developed a method based on Fluorescence Recovery After Photobleaching (FRAP) which allows direct measurement of solute movement in the bone in situ and in real time. Drs. Wang et al. propose to use this approach in combination with mathematical modeling to characterize the solute diffusivity of representative molecules through subchondral bone plate in normal and OA joints. This project will build upon the biological foundation of project #1. Dr. Duncan will serve as mentor.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 最近的实验表明，软骨和骨室之间在体外和体内都有密切的相互作用，其中来自 OA 患者的骨细胞会导致关节软骨基质破裂，并中断与软骨下骨的接触，导致软骨退化。然而，软骨和骨骼相互作用的通讯途径尚不清楚。王博士最近开发了一种基于光漂白后荧光恢复（FRAP）的方法，可以直接原位实时测量骨骼中的溶质运动。博士。王等人。建议使用这种方法与数学模型相结合来表征正常和 OA 关节中代表性分子通过软骨下骨板的溶质扩散率。该项目将建立在项目#1 的生物学基础上。邓肯博士将担任导师。