Core E--Cardiac Morphology, Gene Expression, and Histology

核心E——心脏形态学、基因表达和组织学

• 批准号: 7062844
• 负责人: KENNETH RYAN
• 金额: $ 24.37万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7062844
• 关键词: RNA; Xenopus; biomedical facility; clinical research; congenital heart disorder; gene expression; histology; immunocytochemistry; in situ hybridization; laboratory mouse; morphology

DESCRIPTION (provided by applicant): C1. Services Offered A. Generate and bank frozen stocks of staged mouse embryo RNA samples from E7.5 to E16.5, and of staged Xenopus laevis and Xenopus tropicatis embryo RNA samples from stage 10.5 to stage 46. B. Generate as blocks and sectioned slides, paraffin and OCT embedded mammalian tissues, and mouse and Xenopus embryos. C. Share expertise in and perform some whole mount and section in situ hybridizations using antisense RNA probes. D. Perform immunohistochemistry and tissue histology on explant tissue (Project 1) and embryo sections to examine protein distribution using antibodies to specific proteins.

描述（由申请人提供）： C1。提供的服务 A. 生成并储存 E7.5 至 E16.5 阶段的小鼠胚胎 RNA 样本以及 10.5 至 46 阶段的非洲爪蟾和热带爪蟾胚胎 RNA 样本的冷冻库存。 B. 生成块和切片载玻片、石蜡和 OCT 包埋的哺乳动物组织以及小鼠和非洲爪蟾胚胎。 C. 分享使用反义 RNA 探针进行一些全贴装和切片原位杂交的专业知识并进行这些操作。 D. 对外植体组织（项目 1）和胚胎进行免疫组织化学和组织组织学检查 使用特定蛋白质的抗体检查蛋白质分布的切片。