Effect of antimicrobial nanoemulsions on oral biofilms

抗菌纳米乳对口腔生物膜的影响

• 批准号: 7470812
• 负责人: VALERIE A LEE
• 金额: $ 11.8万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-06-01 至 2013-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7470812
• 关键词: Adult; Affect; Animals; Antimicrobial Effect; Award; Bacteria; Biocompatible Materials; Cell Death; Cell membrane; Charge; Communities; Complex; Condition; Coupled; Dental Hygiene; Dental Plaque; Dental caries; Diet; Disruption; Drug Formulations; Ecology; Edetic Acid; Effectiveness; Emulsions; Environment; Experimental Designs; Exposure to; Fluorescent in Situ Hybridization; General Population; Goals; Growth; Health Professional; In Vitro; Incidence; Investigation; Iron; Laboratories; Lactoferrin; Lasers; Mediating; Mentors; Methods; Microbial Biofilms; Microbiology; Molecular Biology; Oils; Oral; Oral cavity; Oral health; Organism; Osmolar Concentration; Oxygen; Phase; Physiological; Population; Predisposition; Prevention; Rattus; Reproduction spores; Research; Research Personnel; Resistance; Salts; Scanning; Scientist; Series; Streptococcus mutans; Structure; Sucrose; Surface; Techniques; Technology; Testing; Texas; Tooth Diseases; Toxic effect; Universities; Virus; Water; Work; antimicrobial; antimicrobial drug; base; career; fungus; innovation; microorganism; nanoDroplet; nanoemulsion; oral biofilm; permanent tooth; prevent; programs; research study; size; success; surfactant; tooth surface

DESCRIPTION (provided by applicant): The broad objective of the proposed work is to determine optimum nanoemulsion formulations that effectively disrupt or prevent the formation and growth of oral biofilms associated with dental caries, and also to understand the mechanisms involved in their disruption. The incidence of dental caries in the population is still high. The average adult in the U.S. has from 10 to 17 decayed, missing or filled permanent teeth. Nanoemulsions are surfactant-containing oil-in-water emulsions (droplet size 400-800 nm) that are non-toxic to animals but very effective against bacteria, fungi, viruses and spores in their free-floating form. Specific Aim 1: Determine optimum nanoemulsion formulations that prevent the formation and growth of, and disrupt the presence of mixed-organism biofilms in vitro. Specific Aim 2: Investigate the effectiveness of selected nanoemulsions in a study involving rats. Specific Aim 3: Evaluate the in vitro susceptibility of biofilms to nanoemulsions when biofilms are grown under varying physiological conditions likely to be present in the oral cavity (high-low sucrose, high-low iron, high-low osmolarity, high-low oxygen), and also investigate changes in biofilm structure and community profiles. Both S. mutans in pure culture and defined mixed-organism cultures will be investigated. Specific Aim 4: Determine the mechanism of action of the nanoemulsion effect on S. mutans. The proposed work is innovative because the effects of antimicrobial nanoemulsions on biofilms common to the oral cavity have not been investigated. My goal is to be an academic biomaterials scientist with an independent research effort in the treatment and prevention of intra-oral biofilms, and the investigation of mechanisms involved in their disruption. I have successfully found expert collaborators at UTHSCSA and Texas State University for this interdisciplinary project. A K08 award will allow me to acquire the necessary microbiology and molecular biology background and laboratory techniques to successfully complete the proposed work, and will also allow me to utilize the benefits of mentoring during the transition to an independent research career. This research studies a new method of preventing tooth decay. Success may alleviate this problem, which affects a majority of people.

描述（由申请人提供）：所提议工作的主要目标是确定有效破坏或防止与龋齿相关的口腔生物膜形成和生长的最佳纳米乳液配方，并了解破坏这些生物膜的机制。人群中龋齿发病率仍然较高。美国成年人平均有 10 至 17 颗恒牙腐烂、缺失或补过。纳米乳液是含有表面活性剂的水包油乳液（液滴大小 400-800 nm），对动物无毒，但对自由漂浮形式的细菌、真菌、病毒和孢子非常有效。具体目标 1：确定最佳纳米乳液配方，以防止体外混合生物膜的形成和生长并破坏其存在。具体目标 2：在一项涉及大鼠的研究中研究所选纳米乳剂的有效性。具体目标 3：当生物膜在口腔中可能存在的不同生理条件（高-低蔗糖、高-低铁、高-低渗透压、高-低氧）下生长时，评估生物膜对纳米乳液的体外敏感性，并研究生物膜结构和群落概况的变化。将研究纯培养物中的变形链球菌和确定的混合生物体培养物。具体目标 4：确定纳米乳剂对变形链球菌的作用机制。这项工作具有创新性，因为抗菌纳米乳液对口腔常见生物膜的影响尚未得到研究。我的目标是成为一名学术生物材料科学家，在口腔内生物膜的治疗和预防方面进行独立研究，并研究其破坏机制。我已经成功地在 UTHSCSA 和德克萨斯州立大学找到了这个跨学科项目的专家合作者。 K08 奖将使我能够获得必要的微生物学和分子生物学背景以及实验室技术，以成功完成拟议的工作，并且还使我能够在向独立研究职业过渡期间利用指导的好处。这项研究研究了一种预防蛀牙的新方法。成功可能会缓解这个影响大多数人的问题。