Project 2 Genetic Analysis of Somatosensory Cortical Plasticity

项目2 体感皮质可塑性的遗传分析

• 批准号: 7176724
• 负责人: KEVIN FOX
• 金额: $ 28.92万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7176724
• 关键词: Adult; Ally; Alzheimer' s Disease; Animals; Brain Injuries; Cells; Characteristics; Chemosensitization; Chromosome Pairing; Condition; Dendrites; Dendritic Spines; Development; Disease; Genes; Grant; Green Fluorescent Proteins; Laser Scanning Microscopy; Learning; Location; Memory; Memory impairment; Mental Depression; Methods; Mission; Modification; Molecular; Morphology; Mus; Neurons; Pattern; Photons; Process; Property; Public Health; Research Personnel; Screening procedure; Slice; Staging; Stroke; Synapses; Synaptic Transmission; Synaptic plasticity; Testing; Time; Transgenes; Trauma; Vertebral column; Vibrissae; barrel cortex; base; deprivation; experience; functional restoration; genetic analysis; improved; insight; mutant; novel strategies; programs; receptive field; research study; response; size; somatosensory; transmission process

Our long-term objective is to understand the molecular and anatomical basis of cortical plasticity. Closely allied to this aim is to understand whether remote memory is based on cortical plasticity processes and more specifically whether remote memory depends on structurally based cortical plasticity. To these ends we will test two main hypotheses: that Animals showing remote but not short-term memory deficits will show experience-dependent cortical plasticity deficits; Remote memory deficits will be associated with specific deficits in experience-dependent dendritic and spine plasticity. By testing these hypotheses on mutants with deficits in both remote memory and cortical plasticity we will simultaneously begin to reveal the molecular basis of structural cortical plasticity.To test these hypotheses we plan to study four main properties of barrel cortex in animals generated by the remote memory screen: (1) the ability of barrel cortex to undergo experience-dependent plasticity (EDP), (2) normal anatomical and receptive field development of the cortex, (3) spine and bouton stability/turnover in whisker deprived and undeprived animals, (4) excitatory synaptic transmission and the ability to undergo spike-timing dependent plasticity (STOP). By identifying the mechanisms involved in remote memory and cortical plasticity, we may acheive several objectives: the capactiy for modification could be extended in cases of impaired development; insight could be gained into memory and learning deficits in adults; new approaches could be envisioned for restoring cortical function after brain damage. These objectives are therefore directly related to the agency's mission to improve public health because they are aimed at understanding processes that go wrong in disease conditions such as Alzheimer's and restoring function in trauma conditions such as stroke.

我们的长期目标是了解皮质可塑性的分子和解剖基础。密切 与此目标相关的是了解远程记忆是否基于皮质可塑性过程等等 特别是远程存储器是否取决于基于结构的皮质可塑性。到这些目的，我们将 测试两个主要的假设：表现出遥远但短期记忆缺陷的动物将显示依赖经验的皮质可塑性缺陷；远程记忆缺陷将与经验依赖的树突状和脊柱可塑性中的特定缺陷有关。 通过在远程记忆和皮质可塑性中具有不足的突变体上测试这些假设，我们将 同时开始揭示结构皮质可塑性的分子基础，以检验这些假设 我们计划研究由遥控器屏幕产生的动物中枪管皮层的四个主要特性： （1）枪管皮质具有经验依赖性可塑性（EDP）的能力，（2）正常解剖和 皮质的接受田间发展，（3）脊柱稳定性和bout骨稳定性/流失的晶须depover v。 不繁殖的动物，（4）兴奋性突触传播和依赖峰值的能力 可塑性（停止）。 通过识别远程记忆和皮质可塑性所涉及的机制，我们可以达到几种 目标：在发展受损的情况下，可以扩展修改的capactiy；洞察力可以 在成年人中获得记忆和学习缺陷；可以设想恢复新方法 脑损伤后皮质功能。因此，这些目标与该机构的使命直接相关 改善公共卫生，因为它们旨在了解疾病中出错的过程 诸如阿尔茨海默氏症和恢复功能之类的条件在创伤条件（例如中风）中。