Bioanalytical Core

生物分析核心

• 批准号: 7305766
• 负责人: EDWARD J MICHAUD
• 金额: $ 16.64万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-15 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7305766
• 关键词: Amines; Amino Acids; Area; Binding; Biochemical; Biological Assay; Brain; Collaborations; Communities; Complement; Computer Assisted; Computer software; Confocal Microscopy; Consult; Cyclic Nucleotides; DNA; DNA Sequence; Depth; Development; Dialysis procedure; Doctor of Philosophy; Evaluation; Funding; Gene Expression; Genetic; Genotype; Head; High Pressure Liquid Chromatography; Histocytochemistry; Homeostasis; Hormones; Human; Human Development; Human Resources; Image Analysis; In Situ Hybridization; Individual; Lasers; Light; Measures; Messenger RNA; Methodology; Methods; Microdialysis; Microdissection; Modeling; Molecular; Molecular Genetics; Monitor; Mono-S; Mus; Neurons; Neurosciences; Neurosciences Research; Neurotransmitters; Pharmacology; Physical Dialysis; Physiology; Process; Proteins; Psychiatry; Quality Control; RNA Editing; Radioligand Assay; Research; Research Infrastructure; Research Personnel; Research Support; Resolution; Resources; Sampling; Serotonin; Signal Transduction; Synapses; Tail; Techniques; Technology Transfer; Time; Training; Western Blotting; base; brain cell; experience; extracellular; gamma-Aminobutyric Acid; immunocytochemistry; in vivo; member; molecular/cellular imaging; neurochemistry; neurodevelopment; professor; programs; radioligand; receptor; relating to nervous system; tool

R( ROVIDED. he Vanderbilt Conte Center for Neuroscience Research Signaling Networks Supporting Serotonin ransport involves a set of highly interdependent projects targeting genetic, molecular, biochemical and cellular facets of serotonin neuronal identity, development, signaling and physiology. The Bioanalytical Core will provide a set of physical resources accompanied by dedicated technical staff in support of these projects. This core, directed by Dr. Ariel Deutch, Professor of Psychiatry and Pharmacology at Vanderbilt, organizes research support on behalf of Conte Investigators in the areas of 1) Neurochemistry, 2) Cellular and Molecular Imaging, and 3) Neurogenomics. The neurochemical activities include HPLC analyses of amino acid and monoarnine transmitters (e.g. GABA, 5HT, DA) and metabolites, RIAs fo cyclic nucleotides and hormones, radioligand binding of 5HT receptors and synaptosomaltransport assays, RNA/DNA/protein quantitation and in vivo microdialysis to monitor extracellular 5HT. Support for protein analyses includes general support for western blotting from cell and brain extracts. The Cellular and Molecular Imaging division srovides support for in situ hybridization and immunocytochemistry using both light and confocal microscopy as well as automated, high-throughput section processing and computer-assisted quantitation software Laser-assisted microdissection of samples for analysis of raphe mRNA and protein is provided Neurogenomics activities consist of support for DNA/RNA extraction and quantitation, DNA sequencing mouse genotyping, and RNA editing. Dr. Deutch will coordinate and oversee the activities of dedicated support staff, including Mr. Ray Johnson, a highly experienced neurochemist and manager of the Center fo Molecular Neuroscience Neurochemistry Core, who will apportion part of his effort directly in support of the Conte initiative. One to-be-hired staff member of the Core will be a post PhD level associate who will be fully supported for the duration of the project through institutional funds. Dr. Karoly Mimics, an expert in analysis of gene expression in microdissected brain samples will serve as a consultant to the core. Dr. Lyn Daws wi consult with Pis seeking to complement dialysis measures of brain 5HT homeostasis with higher time resolution analysis of SERT activity in vivo. Detailed methods and resources offered by the Core will be f liable at the Contewebsite and distributed freely to the scientific community.

r（狂欢。 他vanderbilt孔戴神经科学研究信号网络支持5-羟色胺 抢劫涉及一组针对遗传，分子，生化和的高度相互依存的项目 5-羟色胺神经元身份，发育，信号传导和生理学的细胞相。生物分析 核心将提供一组物理资源，并由专门的技术人员陪同这些资源来支持这些资源 项目。该核心由范德比尔特精神病学和药理学教授Ariel Deutch博士执导， 代表孔戴研究人员在1）神经化学领域的研究支持，2）细胞 和分子成像，以及3）神经基因组学。神经化学活动包括HPLC分析 氨基酸和单氨基发射机（例如GABA，5HT，DA）和代谢物，RIAS FO循环核苷酸 和激素，5HT受体的放射性结合和突触体级传播测定，RNA/DNA/蛋白 定量和体内微透析以监测细胞外5HT。对蛋白质分析的支持包括 对细胞和脑提取物的蛋白质印迹的一般支持。细胞和分子成像分裂 使用光和共聚焦显微镜，Srovides支持原位杂交和免疫细胞化学化学 以及自动，高通量部分处理和计算机辅助定量软件 提供了样品的激光辅助显微解剖，以分析Raphe mRNA和蛋白质 神经基因组学活性包括对DNA/RNA提取和定量的支持，DNA测序 小鼠基因分型和RNA编辑。 Deutch博士将协调和监督专用的活动 支持人员，包括经验丰富的神经化学家兼中心经理Ray Johnson先生 分子神经科学神经化学核心，他将直接分配他的努力的一部分，以支持 孔戴计划。核心雇用的一位工作人员将是博士后级别的助理 通过机构资金在项目期间得到支持。分析专家Karoly Mimics博士 微解剖的脑样品中的基因表达将作为核心的顾问。 Lyn Daws Wi博士 咨询PI，寻求以更高的时间来补充大脑5HT稳态的透析度量 SERT活性在体内的分辨率分析。核心提供的详细方法和资源将是 f 在Contewebsite上负责并自由分发给科学界。