Cell Culture

细胞培养

基本信息

批准号：
7393283
负责人：
JAMES RHEINWALD
金额：
$ 10.8万
依托单位：
BRIGHAM AND WOMEN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7393283
关键词：
Affect Area Belief Biological Assay Biology Biopsy Birth Boston Cell Line Cells Cellular biology Collection Complex Consultations Cryopreserved Cell Culture Media Cultured Cells Custom Dermal Digital Photography Disease model Equipment Experimental Designs Fibroblasts Growth Inhibitors Human Individual Inherited Instruction Investigation Knockout Mice Life Methods Microscope Mitogens Modeling Molecular Mus Mutant Strains Mice Phase Photography Population Preparation Production Proteins Publishing RNA Range Research Research Personnel Resources Retroviral Vector Sampling Scientist Sensitivity and Specificity Serum Services Skin System Technology Testing Time Tissues Training Transfection Transgenic Organisms base carcinogenesis cell culture collection cell type cellular transduction digital experience immortalized cell in utero in vivo keratinocyte knockout gene melanocyte research study retroviral transduction skills skin disorder vector

项目摘要

During the past five years, the HSDRC Cell Culture Core has assisted the projects of more than 100 investigators by providing services ranging from providing cryopreserved cell lines from our large culture collection, initiating keratinocyte and fibroblast cultures from biopsies of normal and hereditary skin disease tissue, generating immortal cell lines from primary cells using retroviral vectors, providing consultation and training, performing mitogen-sensitivity and specificity experiments, and serving as a center for new collaborative projects. The heavy use of Cell Culture Core resources has reinforced our belief that the research of many established and new skin disease research scientists in the Boston area, as well as all over the U.S and around the world, is aided significantly by a technical and intellectual center that can assist in the planning and execution of cell culture experiments. The considerable expense and the setup and training time necessary to generate high quality cultures of human and murine skin keratinocytes, fibroblasts, and other skin cell types for pilot experiments represents a barrier to entry for many investigators. The main objective of this Core is to overcome this barrier by providing starter samples of culture medium, cell culture methods and training, primary and immortalized Cell lines from its culture collection, and more complex services, including retroviral and adenoviral transduction of cells. Transgenic and gene knockout technology has made the living mouse a preferred model for determining the molecular basis of many skin diseases. In many cases, however, the mutant mice die in utero or soon after birth, such that cultured populations of the affected cell type must be generated to permit complete investigation. Recently published methods for serial expansion and production of spontaneously immortalized lines of mouse epidermal keratinocytes will be added to the Core's repertoire to support transgenic and knockout mouse studies. Another feature of the barrier to cell culture-based experimentation in skin biology is lack of basic microscope and digital photography equipment and skills. Training and access to conventional microscopes and digital cameras, including time-lapse photography, are new components of this core.

在过去的五年中，HSDRC细胞培养核心通过提供服务来帮助100多名研究人员的项目，从提供我们大型培养物收集的冷冻保存细胞系，启动角质形成细胞和成纤维细胞培养物，以及来自正常和遗传性皮肤疾病组织的活检，从而产生替代的细胞和培训疗法的细胞，并培训疗养的细胞，并培训了养育的细胞。实验，并作为新协作项目的中心。细胞培养核心资源的大量使用加强了我们的信念，即对波士顿地区的许多已建立和新的皮肤病研究科学家以及在美国和世界各地的研究都在有助于计划和执行细胞培养实验的技术和知识中心。生成人和鼠皮角质形成细胞，成纤维细胞和其他皮肤细胞类型的高质量培养所需的大量费用以及设置和培训时间，用于试验实验，这是许多研究人员进入的障碍。该核心的主要目的是通过提供培养基，细胞培养方法和训练，其培养物培养物收藏中的主要和不朽的细胞系以及更复杂的服务（包括逆转录病毒和腺病毒的转导细胞）来克服这一障碍。转基因和基因敲除技术使活小鼠成为确定许多皮肤疾病的分子基础的首选模型。但是，在许多情况下，突变小鼠在子宫内死亡或出生后不久，因此必须生成受影响的细胞类型以允许进行全面研究。最近发表的用于串行扩展和生产小鼠表皮角质形成细胞的生产线的方法将添加到核心的库中，以支持转基因和敲除小鼠研究。皮肤生物学基于细胞培养的实验的障碍的另一个特征是缺乏基本显微镜和数字摄影设备和技能。培训和获取传统显微镜和数码相机（包括延时摄影）的访问是该核心的新组成部分。