CORE--: Cell Culture

核心--：细胞培养

• 批准号: 6756251
• 负责人: JAMES RHEINWALD
• 金额: $ 11.25万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6756251
• 关键词: animal tissue; biomedical facility; fibroblasts; growth media; human tissue; keratinocyte; melanocyte; skin; tissue /cell culture; transfection; vascular endothelium

During the past five years, the HSDRC Cell Culture Core has assisted the projects of more than 100 investigators by providing services ranging from providing cryopreserved cell lines from our large culture collection, initiating keratinocyte and fibroblast cultures from biopsies of normal and hereditary skin disease tissue, generating immortal cell lines from primary cells using retroviral vectors, providing consultation and training, performing mitogen-sensitivity and specificity experiments, and serving as a center for new collaborative projects. The heavy use of Cell Culture Core resources has reinforced our belief that the research of many established and new skin disease research scientists in the Boston area, as well as all over the U.S and around the world, is aided significantly by a technical and intellectual center that can assist in the planning and execution of cell culture experiments. The considerable expense and the setup and training time necessary to generate high quality cultures of human and murine skin keratinocytes, fibroblasts, and other skin cell types for pilot experiments represents a barrier to entry for many investigators. The main objective of this Core is to overcome this barrier by providing starter samples of culture medium, cell culture methods and training, primary and immortalized Cell lines from its culture collection, and more complex services, including retroviral and adenoviral transduction of cells. Transgenic and gene knockout technology has made the living mouse a preferred model for determining the molecular basis of many skin diseases. In many cases, however, the mutant mice die in utero or soon after birth, such that cultured populations of the affected cell type must be generated to permit complete investigation. Recently published methods for serial expansion and production of spontaneously immortalized lines of mouse epidermal keratinocytes will be added to the Core's repertoire to support transgenic and knockout mouse studies. Another feature of the barrier to cell culture-based experimentation in skin biology is lack of basic microscope and digital photography equipment and skills. Training and access to conventional microscopes and digital cameras, including time-lapse photography, are new components of this core.

在过去的五年中，HSDRC细胞培养核心通过提供服务范围从我们大型培养物收集中提供冷冻保存的细胞系，启动角质形成细胞和来自正常和遗传性皮肤疾病组织活检的成纤维细胞培养的服务，从而帮助了100多名研究人员的项目。使用逆转录病毒载体从原代细胞产生不朽的细胞系，提供咨询和培训，进行有丝分裂原敏感性和特异性实验，并作为新协作项目的中心。细胞培养的大量使用核心资源加强了我们的信念，即波士顿地区的许多已建立和新的皮肤病研究科学家以及在美国和世界各地的研究都在技术和知识中心得到了很大的帮助这可以帮助计划和执行细胞培养实验。生成人和鼠皮角质形成细胞，成纤维细胞和其他皮肤细胞类型的高质量培养所需的大量费用以及设置和培训时间，用于试验实验，这是许多研究人员进入的障碍。该核心的主要目的是通过提供培养基，细胞培养方法和训练，其培养物培养物收藏中的主要和不朽的细胞系以及更复杂的服务（包括逆转录病毒和腺病毒的转导细胞）来克服这一障碍。转基因和基因敲除技术使活小鼠成为确定许多皮肤疾病的分子基础的首选模型。但是，在许多情况下，突变小鼠在子宫内死亡或出生后不久，因此 必须生成受影响的细胞类型以允许进行全面研究。最近发表的用于串行扩展和生产小鼠表皮角质形成细胞的生产线的方法将添加到核心的库中，以支持转基因和敲除小鼠研究。皮肤生物学基于细胞培养的实验的障碍的另一个特征是缺乏基本显微镜和数字摄影设备和技能。培训和获取传统显微镜和数码相机（包括延时摄影）的访问是该核心的新组成部分。