A cDNA Sequence Resource for Baboon

狒狒 cDNA 序列资源

• 批准号: 7482235
• 负责人: Roger E. Bumgarner
• 金额: $ 57.59万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7482235
• 关键词: Adult; African; Alcoholic Liver Diseases; Alleles; American Type Culture Collection; Animal Model; Animals; Atherosclerosis; Atlases; Bioinformatics; Biological Sciences; Biomedical Research; Biotin; Brain; Brain region; Cerebellum; Characteristics; Chromosome Mapping; Collaborations; Collection; Communities; Comparative Genomic Analysis; Complementary DNA; Computer software; Custom; DNA Microarray Chip; DNA Microarray format; DNA Sequence; Data; Data Analyses; Databases; Deposition; Development; Exhibits; Foundations; Genbank; Gene Expression; Generic Drugs; Genes; Genome; Genome Mappings; Genomics; Goals; Histocompatibility Testing; Homologous Gene; Human; Human Biology; Human Genome; Hybrids; Imagery; Individual; Information Distribution; Institutes; Label; Length; Letters; Libraries; Light; Macaca; Maps; Medicine; Mental disorders; Messenger RNA; Methods; Mitochondrial DNA; Modeling; Molecular; Monitor; Numbers; Oligonucleotides; Organism; Osteoporosis; Pan Genus; Pancreatic ribonuclease; Papio; Papio anubis; Papio hamadryas; Parents; Physiological; Placenta; Positioning Attribute; Primates; Principal Investigator; Process; Proteins; Proteomics; RNA; RNA Splicing; Range; Rate; Relative (related person); Research; Research Design; Research Personnel; Resources; Scientist; Sequence Homology; Signal Transduction; Site; Streptavidin; System; Systems Analysis; Techniques; Technology; Testing; Thalamic structure; Tissues; Transcript; Travel; Universities; Variant; Washington; Work; cDNA Library; comparative; cost; design; design and construction; experience; fetal; frontal lobe; functional genomics; insulin sensitivity; interest; magnetic beads; nonhuman primate; professor; programs; reconstruction; repository; success; tool

DESCRIPTION (Provided by applicant): The primary goal of this application is to generate genomic resources for use by the primate research community and specifically by those researchers performing studies with baboons. At present (June 2005), there are a total of 2496 DNA sequences in Genbank from all baboon species combined. Of these, approximately 1,532 sequences were obtained from Papio anubis (olive baboon) and 639 were obtained from Papio hamadryas (hamadryas baboon). Many of these sequences are redundant - e.g. they represent the same gene (or region of mitochondrial DNA). Hence, there are fewer than 1000 unique baboon gene sequences represented in GenBank. The lack of molecular sequence information for baboon is troubling as the baboon is widely used in biomedical research. Nearly all of these studies will benefit both prospectively and retrospectively from the elaboration of the Baboon genome and related functional genomic studies. Moreover, comparative genomics studies between Baboon and Humans will allow us to determine the relevance of these animal studies to human biology and medicine. Finally, genome data are critical to the application of many functional genomics technologies and to molecular studies in Baboon. In particular, accurate sequence is absolutely necessary for the application of mass spectrometric methods of protein identification and to the design of DNA microarrays. In this application we propose to 1) Create normalized cDNA libraries from placenta, fetal brain and three regions of adult brain, 2) Sequence approximately 400,000 total clones 3) Create a database of annotated sequences and primate homologues, map the sequences to the human and chimp assemblies and identify likely SNP's in our sequences and 4) Create publicly available microarray designs for use by the baboon research community.

描述（由申请人提供）： 该应用程序的主要目标是生成基因组资源，以供灵长类动物研究界，特别是那些用狒狒进行研究的研究人员。目前（2005年6月），来自所有狒狒种类的GenBank总共有2496个DNA序列。其中，从帕皮奥·阿比斯（Papio Anubis）（橄榄狒狒）获得了大约1,532个序列，并从帕皮奥·哈马德里斯（Papio Hamadryas）（hamadryas baboon）获得了639个序列。这些序列中的许多是多余的 - 例如它们代表相同的基因（或线粒体DNA的区域）。因此，GenBank中代表的独特狒狒基因序列少于1000个。狒狒缺乏分子序列信息令人不安，因为狒狒被广泛用于生物医学研究中。几乎所有这些研究都将从狒狒基因组和相关功能基因组研究的阐述中前瞻性和追溯性地受益。 此外，狒狒和人类之间的比较基因组学研究将使我们能够确定这些动物研究与人类生物学和医学的相关性。 最后，基因组数据对于许多功能基因组技术的应用至关重要，对狒狒的分子研究至关重要。 特别是，对于应用蛋白质鉴定的质谱方法和DNA微阵列的设计，准确的序列是绝对必要的。 在此应用中，我们建议1）从胎盘，胎儿大脑和成人大脑的三个区域创建标准化的cDNA文库，2）序列约400,000个总克隆3）创建一个带注释的序列的数据库和Primate同源物，将序列映射到人类和杂物组件中，并在我们的序列中确定了SNP，并在我们的序列中确定了公共场所的使用，以创建Micm Mictor Array Array的设计。