Title Omitted

标题省略

• 批准号: 7360578
• 负责人: JUDITH L YANOWITZ
• 金额: $ 10.52万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-15 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7360578
• 关键词: Address; Affect; Age; Aging; Aneuploidy; Animals; Behavior; Biological Assay; Biological Models; Caenorhabditis elegans; Candidate Disease Gene; Cell Nucleus; Cell division; Chromatin Structure; Chromosome Segregation; Chromosomes; Clinical; Cues; DNA biosynthesis; Defect; Development; Diploidy; Down Syndrome; Ensure; Environment; Enzymes; Event; Fellowship; Female; Fostering; Functional disorder; Future; Gene Conversion; Genes; Genetic; Genetic Materials; Genetic Nondisjunction; Genetic Recombination; Germ Cells; Germ Lines; Haploidy; Homologous Gene; Human; Imagery; Institutes; Institution; Invaded; Invertebrates; Knowledge; Lead; Length; Life; Longevity; Meiosis; Meiotic Prophase I; Meiotic Recombination; Meta-Analysis; Molecular; Molecular Genetics; Monosomy; National Institute of Diabetes and Digestive and Kidney Diseases; Nematoda; Oocytes; Pathway interactions; Postdoctoral Fellow; Principal Investigator; Process; Production; Program Development; Proteins; Proteomics; Protocols documentation; Purpose; Reproduction; Research; Resected; Role; Running; Scientist; Signal Transduction; Sister Chromatid; Sperm Count Procedure; Synaptonemal Complex; System; Techniques; Testing; Therapeutic; Tissues; Title; Topoisomerase; Training; Training Programs; Trisomy; Universities; Washington; Woman; age effect; age related; aging gene; career; cellular imaging; cohesion; desire; egg; male; mutant; neuronal cell body; normal aging; programs; repaired; reproductive; scaffold

DESCRIPTION (provided by applicant): This proposal describes a training program for the development of career in aging research. The PI completed a post-doctoral fellowship in C. elegans genetics, and for the last three years has run a small lab studying meiotic recombination. This proposal addresses the Pi's desire to develop a new program of research within her lab, taking the expertise she already has on meiosis and applying it to the study of reproductive aging. The training program will draw on the expertise of Drs. Catherine Wolkow of the National Institute of Aging and Kerry Kornfeld of Washington University, who actively study different aspects of C. elegans aging, and Dr. Doug Koshland of the Carnegie Institution who is a leader in the field chromosome behavior. In addition, Dr. Andy Golden of NIDDK and Dr. Valerie Reinke will provide technical training in live cell imaging of meiosis and of microarrays, respectively. The research focuses on reproductive decline associated with aging in the nematode, C. elegans. Defects in chromosome segregation during gamete formation appear with age in both C. elegans and humans. In females, this leads to a significant increase (>15-fold) in aneuploid oocytes by age 40, and in males, a decrease in sperm count. Despite the clinical importance of research in this field, little is known about the underlying molecular mechanisms responsible for changes in the fidelity of meiotic chromosome segregation. The prodigous progress made in the fields of aging and meiosis by research in C. elegans underscores the use of this system to address fundamental questions related to germ line aging. The specific aims include: 1) characterizing meiosis during normal aging in C. elegans; 2) Analyzing aging pathways for their role in germ line aging; and 3) identifying germ line genes that respond to aging cues. The Carnegie Institution provide a superb setting for the training of young scientists by creating an environment that fosters interaction between scientists and by providing opportunities for professional development. In addition, the proximity of Carnegie to the National Institute of Aging will provide additional opportunities for the PI to interact with scientists in the aging field through participation in a local invertebrate aging research group and attendance at seminars on that campus. This training program should allow the PI to establish a strong research program in the aging field as she prepares for her future academic career.

描述（由申请人提供）：该提案描述了一项培训计划，以发展衰老研究的职业。 PI完成了秀丽隐杆线虫遗传学的博士后研究金，在过去的三年中，一个小型实验室研究减数分裂重组。该提案旨在解决PI在她的实验室内开发新的研究计划的愿望，以她对减数分裂的专业知识并将其应用于生殖衰老的研究。该培训计划将借鉴DRS的专业知识。美国国家老化研究所的凯瑟琳·沃尔科（Catherine Wolkow）和华盛顿大学的克里·科恩菲尔德（Kerry Kornfeld），他们积极研究秀丽隐杆线虫衰老的不同方面，以及卡内基机构的道格·科什兰（Doug Koshland）博士，他们是现场染色体行为的领导者。此外，Niddk的Andy Golden博士和Valerie Reinke博士将分别提供减数分裂和微阵列的活细胞成像的技术培训。该研究的重点是与线虫秀丽隐杆线虫中衰老相关的生殖下降。配子形成过程中染色体隔离的缺陷在秀丽隐杆线虫和人类中都随着年龄的增长而出现。在女性中，这会导致到40岁的非整倍体卵母细胞和男性中的非整倍体卵母细胞的显着增加（> 15倍）。尽管研究在该领域的临床重要性，但对于导致减数分裂染色体隔离的忠诚度变化的基本分子机制知之甚少。秀丽隐杆线虫在衰老和减数分裂领域取得的巨大进步强调了该系统的使用来解决与细菌衰老有关的基本问题。具体目的包括：1）表征秀丽隐杆线虫正常衰老期间的减数分裂； 2）分析衰老途径在生殖系衰老中的作用； 3）确定对衰老提示反应的生殖系基因。卡内基机构通过创造一个环境来培养科学家之间的互动并通过提供专业发展的机会，为培训年轻科学家提供了一流的环境。此外，卡内基与美国国家老化研究所的接近度将为PI提供更多机会，可以通过参加当地的无脊椎动物老化研究小组与衰老领域的科学家进行互动，并参加该校园的研讨会。该培训计划应允许PI在为未来的学术生涯做准备时，在衰老领域建立强大的研究计划。