Mouse Breeding and Husbandry

小鼠饲养及饲养

• 批准号: 7406637
• 负责人: FRANCIS J CASTELLINO
• 金额: $ 18.1万
• 依托单位: UNIVERSITY OF NOTRE DAME
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406637
• 关键词: Animals; Breeding; Complex; Gene Targeting; Genes; Genotype; Housing; Individual; Investigation; Lead; Mus; Numbers; Pathologist; Policies; Probability; Program Research Project Grants; Research; Research Personnel; Supervision; Transgenes; Transgenic Mice; Veterinarians; animal care; day; genetic strain

The Mouse Breeding Core (Core C) will generate, house, and maintain the final breeders needed to obtain the single and combined gene targeted and transgenic mice needed for all projects of the Program Project Grant (PPG). Once obtained, these animals will be turned over to individual investigators of the projects of the PPG for large-scale breedings to generate the required numbers of animals for the studies proposed. The animal complex is directed by a veterinarian and board certified veterinary pathologist, Dr. Mark A. Suckow, who establishes the policies regarding animal care and breeding. An associate director manages day-to-day supervision of the ten technologists who care for the animals and establish the requested breedings. Three of these latter individuals are assigned to animals of the W.M. Keck Center for Transgene Research that will be used in the investigations of the current PPG. The responsibilities of this core will be to generate appropriate mice that when bred by investigators will lead to a high statistical probability of obtaining the specific animals needed for the studies. Given the complexities of the genetic strains of the final animals in these projects, strains with low probabilities of generating the final animals will be first required. Core C will assume responsibility for this latter task. The aims of this core are to generate at a minimum: 1) X[+/-] or X[-/-] (when mice with this genotype are able to survive and breed) mice and X+/-/Y-/- (where Y is a second gene) or X-/-/Y-/- for large-scale breedings to generate animals required for Project by Castellino; 2) APC[Min+/-o]/X+/- (or X-/-, if they survive and breed) mice for large-scale breedings for Project by Ploplis; and 3) to generate sufficient numbers of WT and FX+/- breeders for Project by Rosen.

小鼠育种核心（Core C）将生成，容纳和维护最终的育种者，以获取计划项目赠款（PPG）所有项目所需的单一基因和转基因小鼠所需的最终育种者。一旦获得，这些动物将被移交给PPG项目的个别研究人员，以供大规模繁殖，以生成所需的研究所需的动物数量。该动物综合体由兽医和董事会认证的兽医病理学家Mark A. Suckow博士指导，他制定了有关动物护理和育种的政策。副主任管理十位关心动物并建立要求的繁殖的技术人员的日常监督。这些后者的三个人分配给W.M.的动物。凯克转基因研究中心将用于当前PPG的研究。该核心的责任将是生成适当的小鼠，当研究人员繁殖时，将导致较高的统计概率获得研究所需的特定动物。考虑到这些项目中最终动物的遗传菌株的复杂性，概率低的菌株 首先需要产生最终动物。核心C将对后一种任务承担责任。该核心的目的是至少生成：1）x [+/-]或x [ - / - ]（当具有该基因型的小鼠能够生存并繁殖的小鼠时）小鼠以及x+/ - /y - / - （y是第二个基因）或x - /y - /y - / - 用于生成大型繁殖的动物，以便通过Castellino进行投射; 2）apc [min +/- o]/x +/-（或x - / - ，如果它们幸存下来并繁殖）小鼠用于大规模繁殖，用于Ploplis的项目； 3）为Rosen生成足够数量的WT和FX +/-育种者。