Multiphoton Imaging

多光子成像

• 批准号: 7047423
• 负责人: SUSETTE C MUELLER
• 金额: $ 50万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2008-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047423
• 关键词: microscopy; neoplasm /cancer; schools; training

DESCRIPTION (provided by applicant): We request funds towards the purchase of a Zeiss510/ NLO microscope with Ti:sapphire Chameleon laser. Five principle investigators from the Medical School comprise the major user group (Faden, Mueller, Pestell, Roman, Van Keuren) and 8 minor users are described including faculty from the Medical Center and the Undergraduate Campus. A total of 11 NIH-funded investigators will be supported by this application. Projects conducted by major users include: vital imaging of invasion in tissues by tumor or transgenic mammary epithelial cells; vital imaging in situ of mitochondrial function in liver; imaging temperature sensitive probes in skin; and imaging vasculogenesis in zebrafish. Minor projects include imaging the effects of stable chemotactic gradients on neuronal growth cones embedded in thick collagen gels; vital imaging of neurons and parasite infection in the gut; trafficking of G protein-coupled receptors in hypertension; live imaging of vacuolar pH within living malarial parasites; and supporting the research and educational mission of the Lombardi Comprehensive Cancer Center (LCCC) Microscopy and Imaging Shared Resource (MISR). The new microscope will be housed in the LCCC MISR. The Principle Investigator, Dr. Mueller, is an NIH funded tumor cell biologist and has been director of this facility since 1991. LCCC and Georgetown University have committed space, additional funding to meet the total purchase price and yearly service contract dollars to support the acquisition and maintenance of this instrument. Members of the major user group with microscopy expertise including the PI (Mueller) and a physicist with multiphoton expertise (Van Keuren) will be trained initially together with one of our full time MISR microscopists (Palchik). Mr. Palchik who has expertise in confocal and digital light microscopy will support use and training under the direction of Drs. Mueller and Van Keuren. The shared resource will perform associated administrative tasks including billing. Oversight will be provided by our 8 member advisory committee. In 2004, the Shared Resource was utilized by 60 investigators representing 15 departments in the Medical School and the Undergraduate Campus. MISR has an outstanding record in training. Each year since 1999, we have trained between 30- 40 LCC investigators to use the existing microscopes in the MISR. The Shared Resource is maintained by a combination of Cancer Center and grant support. Users contribute to the resource in the form of user charge back fees. There is no multiphoton microscope at Georgetown and animal imaging activities are severely curtailed as a result.

描述（由申请人提供）： 我们请求资金购买配备 Ti:sapphire Chameleon 激光器的 Zeiss510/ NLO 显微镜。来自医学院的五位主要研究人员组成了主要用户组（Faden、Mueller、Pestell、Roman、Van Keuren），描述了 8 名次要用户，包括来自医学中心和本科校园的教职人员。该申请将支持总共 11 名 NIH 资助的研究人员。主要用户开展的项目包括：肿瘤或转基因乳腺上皮细胞侵入组织的生命成像；肝脏线粒体功能的原位生命成像；对皮肤中的温度敏感探头进行成像；以及斑马鱼血管生成的成像。小项目包括对稳定趋化梯度对嵌入厚胶原凝胶中的神经元生长锥的影响进行成像；神经元和肠道寄生虫感染的重要成像；高血压中 G 蛋白偶联受体的运输；活体疟疾寄生虫内液泡 pH 值的实时成像；支持隆巴迪综合癌症中心 (LCCC) 显微镜和成像共享资源 (MISR) 的研究和教育使命。新显微镜将安装在 LCCC MISR 中。首席研究员 Mueller 博士是一位 NIH 资助的肿瘤细胞生物学家，自 1991 年以来一直担任该设施的主任。LCCC 和乔治敦大学已承诺提供空间、额外资金以满足总购买价以及年度服务合同美元来支持收购以及本仪器的维护。具有显微镜专业知识的主要用户组成员，包括 PI (Mueller) 和具有多光子专业知识的物理学家 (Van Keuren)，最初将与我们的一位全职 MISR 显微镜专家 (Palchik) 一起接受培训。 Palchik 先生拥有共焦和数字光学显微镜方面的专业知识，他将在 Drs. 的指导下支持使用和培训。穆勒和范科伦。共享资源将执行相关的管理任务，包括计费。我们的 8 名成员咨询委员会将提供监督。 2004 年，代表医学院和本科生校区 15 个系的 60 名研究人员使用了共享资源。 MISR 在培训方面有着出色的记录。自 1999 年以来，我们每年都会培训 30-40 名 LCC 研究人员使用 MISR 中的现有显微镜。共享资源由癌症中心和赠款支持共同维护。用户以用户退款的形式贡献资源。乔治城没有多光子显微镜，动物成像活动因此受到严重限制。