CORE--MICROSCOPY AND IMAGING FACILITY

核心——显微镜和成像设备

• 批准号: 6203167
• 负责人: SUSETTE C MUELLER
• 金额: $ 7.93万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-30 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203167
• 关键词: bioimaging /biomedical imaging; biomedical facility; electron microscopy; light microscopy; microinjections; neoplasm /cancer

Microscopy and Imaging provides instrumentation, training, and expert advice for light and electron microscopy d image analysis. Light microscopy includes optics for bright field, dark field, phase contrast, differential interference contrast or Hoffman optics, epifluorescence wide field and confocal microscopy for high resolution light microscopy of tumor cells, tissues, and subcellular localizations. Transmission and scanning electron microscopes available for high resolution subcellular morphology. A newly acquired microinjection system allows us to microinject individual tumor cells. The inverted microscope on this system is interfaced with a camera for digitizing images. The installation of Windows-compatible Optimas 5.1 updates our capacity for image analysis. Time lapse imaging of motile cells is now underway using this work station. The shared facility has a greatly expanded capacity or image storage and transfer with the recent acquisition of writable CD-ROM and networking of the digitized imaging work stations. In addition to traditional studies supported by the core, some examples of recent projects the image analysis/ microinjection system include microinjection of plasmids and antisense oligonucleotides, quantification of nuclear size and shape, in vitro angiogenesis, live cell motility, size and extent of epithelial branching in developing mouse mammary glands, and cell surface structure. Dr. Susette Mueller is the Director and Manager of the Shared Resource, and Dr. Maozheng Dai is the Microscopist. The main laboratory of the shared source is now located on the fourth floor in the Cancer Center space within the new Research Building. The electron and confocal microscopes are m their original location in the adjacent Preclinical Science Building. The use of the facility has expanded significantly in the past three years, with about double the number of principle investigators (15 to 33), peer-reviewed projects (15 to 34), and departments (8 to 13) utilizing the Shared Resource. The Resource in-acts with all 8 programs and with 4 other Shared Resources.

显微镜和成像提供仪器、培训和 光学和电子显微镜 d 图像分析的专家建议。 光学显微镜包括用于明场、暗场、 相衬、微分干涉衬度或霍夫曼 光学、落射荧光宽视野和共焦显微镜，用于高 肿瘤细胞、组织和细胞的高分辨率光学显微镜 亚细胞定位。透射电子和扫描电子 显微镜可用于高分辨率亚细胞形态。 新获得的显微注射系统使我们能够进行显微注射 单个肿瘤细胞。该系统上的倒置显微镜是 与相机连接以数字化图像。安装 兼容 Windows 的 Optimas 5.1 更新了我们的图像容量 分析。目前正在使用运动细胞的延时成像 这个工作站。共享设施大大扩展 最近采集的容量或图像存储和传输 可写CD-ROM和数字化成像工作的网络化 站。除了核心支持的传统研究之外， 最近项目的一些例子图像分析/显微注射 系统包括质粒和反义的显微注射 寡核苷酸，核大小和形状的量化， 体外血管生成、活细胞运动、大小和范围 发育中的小鼠乳腺的上皮分支和细胞 表面结构。 Susette Mueller 博士担任董事兼经理 戴茂正博士是共享资源的显微镜师。 共享源的主要实验室现位于 新研究中心癌症中心四楼空间 建筑。电子显微镜和共焦显微镜是他们的 原来的位置在邻近的临床前科学大楼。这 过去三年来，该设施的使用量显着扩大 年，主要研究人员的数量大约增加了一倍（15 至 33）、同行评审项目（15 至 34）和部门（8 至 34） 13）利用共享资源。该资源与所有 8 个一起起作用 程序和其他 4 个共享资源。