CORE--PRIMATE

核心--灵长类

• 批准号: 7575005
• 负责人: KENNETH CAREY
• 金额: $ 16.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7575005
• 关键词: 1 year old; Acute; Adolescent; Aftercare; Age; Animals; Antiatherogenic; Apolipoprotein A-I; Atherosclerosis; Autopsy; Biomedical Research; Blood Vessels; Blood specimen; Body Weight; Buffers; Chemistry; Containment; Control Animal; Defect; Depressed mood; Dialysis procedure; Dichloromethylene Diphosphonate; Diet; Dose; End Point; Exposure to; Fetoprotein; Formalin; Foundations; Gene Expression; Gene Transfer; Genes; Hepatic; High Density Lipoproteins; Hour; Human; Inflammatory Response; Injection of therapeutic agent; Ketamine; Kinetics; Kupffer Cells; LDLR gene; Lesion; Lipids; Lipoproteins; Liposomes; Low-Density Lipoproteins; Macaca mulatta; Measures; Mediating; Medicine; Monitor; Papio; Paraffin Embedding; Phenotype; Physical Dialysis; Physiology; Polymers; Primates; Protein Overexpression; Protocols documentation; Retreatment; Safety; Sampling; Serum; Severities; Shipping; Ships; Testing; Texas; Time; Tissues; Toxic effect; Training; Virus; Virus Shedding; Week; college; day; experience; gene therapy; improved; liver biopsy; nonhuman primate; protein expression; receptor; response; sex; vector

The Gene Therapy Primate Core will be located in the Department of Physiology and Medicine at the Southwest Foundation for Biomedical Research in San Antonio Texas. The core will be responsible for supplying LDLR defective rhesus monkeys and baboons with described lipoprotein phenotypes, administering experimental treatments, maintaining subjects that are shedding virus under appropriate containment (biosafety level 2), sampling animals to measure gene expression and iipoprotein kinetics, necropsying animals to gather tissues to measure final endpoints, assessing vascular lesions in control and treated animals, and preparing and shipping samples to Baylor College of Medicine in Houston, Texas.

基因治疗灵长类动物核心将位于德克萨斯州圣安东尼奥市西南生物医学研究基金会的生理学和医学系。该核心将负责提供具有所述脂蛋白表型的 LDLR 缺陷恒河猴和狒狒，进行实验治疗，将正在排出病毒的受试者维持在适当的遏制下（生物安全级别 2），对动物进行取样以测量基因表达和脂蛋白动力学，对动物进行尸检以收集组织来测量最终终点，评估对照和治疗动物的血管病变，并准备样本并将其运送到德克萨斯州休斯顿的贝勒医学院。