Mouse Animal Models Core

小鼠动物模型核心

基本信息

批准号：
7485190
负责人：
AMANDA J ROBERTS
金额：
$ 30.96万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-27 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7485190
关键词：
Abstinence Adenylate Cyclase Affect Alcohol abuse Alcohol consumption Alcoholism Alcohols Amygdaloid structure Animal Model Animals Appendix Behavioral Behavioral Model Boutos Brain Breeding CRF receptor type 1 CRF receptor type 2 Chronic Colorado Consultations Consumption Data Dependence Development Disease End Point Ensure Environment Ethanol Ethanol dependence Funding GALR1 Galanin Receptor GALR2 Galanin Receptor GALR2 gene Genes Genetic Genetic Models Goals Heavy Drinking Heterozygote Image Individual Intoxication Investigation Investments Knock-out Laboratories Letters Methods Modeling Molecular Mouse Strains Mus Negative Reinforcements Neurobiology Neurosciences Numbers Participant Phenotype Physiological Positive Reinforcements Predisposition Preparation Principal Investigator Procedures Process Production Purpose RNA Interference Range Rattus Research Design Research Personnel Resources Role Running Schedule Self Administration Services Site Slice Staging Standardization System Testing Texas Time Transgenic Model Transgenic Organisms Travel Withdrawal addiction alcohol research alcohol sensitivity alcoholism/alcohol abuse base brain tissue design drinking innovation kappa opioid receptors mouse model mu opioid receptors multidisciplinary neurochemistry neuroimaging novel novel strategies programs tool vapor

项目摘要

DESCRIPTION (provided by applicant): Alcoholism is characterized by increased ethanol intake, loss of control over ethanol intake, and compulsive ethanol taking. Progress in understanding the neurobiological basis of excessive ethanol drinking depends on the combined development and use of molecular and neuropharmacological tools for understanding the mechanisms of ethanol actions and animal models that allow interpretation of these advances in the context of ethanol addiction. The primary goal of the Mouse Animal Models Core is to provide well characterized and validated behavioral models of excessive ethanol consumption to INIA investigators. This Core has been completely designed around the specific needs of the INIA-West group and has developed from intense discussions and contact with everyone. Inherent in the goal of the Core is that it allows for both the standardization of these models and the pooling of resources. This Core will service twelve INIA investigators studying each of the two domains (Binge and Dependence, see below) and their three levels of analysis (neurocircuitry, cellular, and molecular). In this way, the Core shares the responsibility of making this consortium highly integrated and multidisciplinary and establishes economies of scale. Individual laboratories can focus on their own expertise and will not be required to establish these models in their own laboratories. One of the most innovative aspects of this Core is the application of its neuroadaptive models to the many genetic models (transgenics, knockouts, selectively bred mice) being studied and/or produced by INIA investigators. This will allow for a comprehensive study of the combined impact of genes and environment on the process of ethanol addiction and represents a major strength of the present application. In addition, these models will be extended to additional strains such as those used in the production of knockout and transgenic strains and those commonly used in ethanol research. Finally, this Core will examine phenotypic correlates of excessive alcohol consumption as this is critical to both our understanding of the susceptibility. Based on INIA investigators' needs the following Specific Aims have been formulated: Specific Aim 1. To provide established and refined neuroadaptive models to INIA investigators and Specific Aim 2. To combine neuroadaptive models with genetic models for INIA investigators.

描述（由申请人提供）：酒精中毒的特征是乙醇摄入量的增加，对乙醇摄入的控制丧失以及强迫性乙醇。理解过度乙醇饮用的神经生物学基础的进展取决于分子和神经药理学工具的联合发展和使用，以理解乙醇作用和动物模型的机制，这些机制允许在乙醇成瘾的背景下解释这些进步。小鼠动物模型核心的主要目标是向INIA研究者提供过度表征和验证的过度乙醇消耗的行为模型。该核心已完全围绕INIA-West Group的特定需求而设计，并从激烈的讨论和与所有人接触而发展。核心目标固有的是，它允许这些模型的标准化和资源的汇总。该核心将为研究两个领域中的每个域（暴饮暴食和依赖，见下文）及其三个分析水平（神经循环，细胞和分子）的研究人员提供服务。通过这种方式，核心具有使该财团高度融合和多学科的责任，并建立了规模经济。个别实验室可以专注于自己的专业知识，并且不需要在自己的实验室中建立这些模型。该核心最具创新性的方面之一是将其神经适应性模型应用于INIA研究人员正在研究和/或生产的许多遗传模型（转基因，敲除，有选择性的繁殖小鼠）。这将允许对基因和环境对乙醇成瘾过程的综合影响进行全面研究，并代表当前应用的主要优势。此外，这些模型将扩展到其他菌株，例如用于产生敲除和转基因菌株以及通常用于乙醇研究的菌株。最后，该核心将检查过度饮酒的表型相关性，因为这对于我们对易感性的理解至关重要。根据INIA研究人员的需求，已经制定了以下特定目标：具体目的1。为INIA研究者提供既定和精致的神经适应性模型，并具体目的2。将神经适应性模型与INIA研究者的遗传模型相结合。