Mouse Animal Models Core

小鼠动物模型核心

基本信息

批准号：
7291090
负责人：
AMANDA J ROBERTS
金额：
$ 30.57万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-27 至 2011-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7291090
关键词：
Abstinence Adenylate Cyclase Affect Alcohol abuse Alcohol consumption Alcoholism Alcohols Amygdaloid structure Animal Model Animals Appendix Behavioral Behavioral Model Boutos Brain Breeding CRF receptor type 1 CRF receptor type 2 Chronic Colorado Consultations Consumption Data Dependence Development Disease End Point Ensure Environment Ethanol Ethanol dependence Funding GALR1 Galanin Receptor GALR2 Galanin Receptor GALR2 gene Genes Genetic Genetic Models Goals Heavy Drinking Heterozygote Image Individual Intoxication Investigation Investments Knock-out Laboratories Letters Methods Modeling Molecular Mouse Strains Mus Negative Reinforcements Neurobiology Neurosciences Numbers Participant Phenotype Physiological Positive Reinforcements Predisposition Preparation Principal Investigator Procedures Process Production Purpose RNA Interference Range Rattus Research Design Research Personnel Resources Role Running Schedule Self Administration Services Site Slice Staging Standardization System Testing Texas Time Transgenic Model Transgenic Organisms Travel Withdrawal addiction alcohol research alcohol sensitivity alcoholism/alcohol abuse base brain tissue design drinking innovation kappa opioid receptors mouse model mu opioid receptors multidisciplinary neurochemistry neuroimaging novel novel strategies programs tool vapor

项目摘要

DESCRIPTION (provided by applicant): Alcoholism is characterized by increased ethanol intake, loss of control over ethanol intake, and compulsive ethanol taking. Progress in understanding the neurobiological basis of excessive ethanol drinking depends on the combined development and use of molecular and neuropharmacological tools for understanding the mechanisms of ethanol actions and animal models that allow interpretation of these advances in the context of ethanol addiction. The primary goal of the Mouse Animal Models Core is to provide well characterized and validated behavioral models of excessive ethanol consumption to INIA investigators. This Core has been completely designed around the specific needs of the INIA-West group and has developed from intense discussions and contact with everyone. Inherent in the goal of the Core is that it allows for both the standardization of these models and the pooling of resources. This Core will service twelve INIA investigators studying each of the two domains (Binge and Dependence, see below) and their three levels of analysis (neurocircuitry, cellular, and molecular). In this way, the Core shares the responsibility of making this consortium highly integrated and multidisciplinary and establishes economies of scale. Individual laboratories can focus on their own expertise and will not be required to establish these models in their own laboratories. One of the most innovative aspects of this Core is the application of its neuroadaptive models to the many genetic models (transgenics, knockouts, selectively bred mice) being studied and/or produced by INIA investigators. This will allow for a comprehensive study of the combined impact of genes and environment on the process of ethanol addiction and represents a major strength of the present application. In addition, these models will be extended to additional strains such as those used in the production of knockout and transgenic strains and those commonly used in ethanol research. Finally, this Core will examine phenotypic correlates of excessive alcohol consumption as this is critical to both our understanding of the susceptibility. Based on INIA investigators' needs the following Specific Aims have been formulated: Specific Aim 1. To provide established and refined neuroadaptive models to INIA investigators and Specific Aim 2. To combine neuroadaptive models with genetic models for INIA investigators.

描述（由申请人提供）：酗酒的特征是乙醇摄入量增加、对乙醇摄入失去控制以及强迫性摄入乙醇。了解过量乙醇的神经生物学基础的进展取决于分子和神经药理学工具的联合开发和使用，以了解乙醇的作用机制和动物模型，从而在乙醇成瘾的背景下解释这些进展。小鼠动物模型核心的主要目标是为 INIA 研究人员提供经过充分表征和验证的过量乙醇消耗行为模型。该核心完全是围绕 INIA-West 集团的具体需求而设计的，是在与每个人的激烈讨论和接触中发展而来的。核心目标的本质是它允许这些模型的标准化和资源池化。该核心将为 12 名 INIA 研究人员提供服务，研究两个领域（狂欢和依赖性，见下文）及其三个分析水平（神经回路、细胞和分子）。通过这种方式，核心共同承担使这个联盟高度集成和多学科的责任，并建立规模经济。各个实验室可以专注于自己的专业知识，不需要在自己的实验室中建立这些模型。该核心最具创新性的方面之一是将其神经适应性模型应用于 INIA 研究人员正在研究和/或制作的许多遗传模型（转基因、基因敲除、选择性繁殖小鼠）。这将允许全面研究基因和环境对乙醇成瘾过程的综合影响，并且代表了本申请的主要优势。此外，这些模型将扩展到其他菌株，例如用于生产敲除和转基因菌株的菌株以及乙醇研究中常用的菌株。最后，该核心将检查过量饮酒的表型相关性，因为这对于我们了解易感性至关重要。根据 INIA 研究者的需求，制定了以下具体目标：具体目标 1. 为 INIA 研究者提供已建立和完善的神经适应性模型；具体目标 2. 将神经适应性模型与遗传模型相结合，为 INIA 研究者提供服务。