Roche Real-Time Polymerase Chain Reaction Workflow System

罗氏实时聚合酶链式反应工作流程系统

• 批准号: 7388504
• 负责人: Mary Vore
• 金额: $ 12万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-01 至 2009-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388504
• 关键词: Address; Anatomy; Base Sequence; Biological; Biomedical Research; Computer software; DNA; DNA Repair; Detection; Drug Regulations; Effectiveness; Faculty; Funding; Gene Expression; Gene Expression Regulation; Genes; Goals; Grant; Hormones; Hour; Housing; Injury; Interdisciplinary Study; Malignant Neoplasms; Methods; Minor; Mutagenesis; Neurobiology; Nucleic Acids; Nucleic acid sequencing; Oxidants; Polymerase Chain Reaction; Postdoctoral Fellow; Public Health; RNA analysis; Research; Research Personnel; Research Project Grants; Resources; Sampling; Science; Single Nucleotide Polymorphism; Stress; Students; System; Technology; Time; Tissues; Today; Toxicology; Training; Transcript; Universities; Women' s Health; health science research; hormone regulation; improved; instrument; nucleic acid quantitation; research facility; tissue preparation; tumor

DESCRIPTION (provided by applicant): We are requesting a Roche real-time polymerase chain reaction (PCR) Workflow System with automated components for preparation of tissues (MagNA Lyser), isolation of nucleic acids (MagNA Pure Compact), and a real-time PCR (LightCycler 480). The LightCycler 480 is a high throughput real- time PCR instrument that detects and quantifies nucleic acid sequences, and has a 96- and 384-well plate capacity. This PCR Workflow System is very easy to use, is accompanied by powerful software, and enables an investigator to go from tissue to nucleic acid quantitation in 3-4 hours. This instrument will be housed in the MicroArray Core Research Facility of the University in the Bosomworth Health Sciences Research Building, and will be used by faculty in the Graduate Center for Toxicology, and in the Department of Anatomy and Neurobiology. The group of investigators participating in this application includes seven faculty with NIH-supported grant projects who are major users, and three additional faculty who are minor users. The research projects are diverse, but revolve around three major themes: 1) oxidant-stress induced tissue injury and cancer, 2) DNA damage, repair and mutagenesis and 3) hormonal regulation of gene expression. We intend to use this instrument for the high-throughput real-time quantitative analysis of RNA transcripts and DNA targets in biological samples and in detection of single nucleotide polymorphisms (SNP) in genes important in cancer. As indicated in the research projects from each investigator, the availability of the proposed instrument will significantly improve the scope and timeliness of their studies. Most importantly, it will allow the science to determine the pursuit of exciting discoveries in their funded research projects, rather than availability of current instruments. The overall goals of this shared multidisciplinary research facility are to use this advanced technology resource to carry out cutting-edge research that characterizes today's biomedical research and to train our doctoral students and postdoctoral fellows in the use of these modern methods that will enable them to develop into successful independent investigators. The research problems addressed by our investigators are highly relevant to major public health problems related to cancer, both in the mechanisms of tumor formation, the reasons for (lack of) effectiveness of cancer chemotherapeutic drugs, and the regulation of genes by hormones that are important in women's health.

描述（由申请人提供）：我们请求Roche实时聚合酶链反应（PCR）工作流程系统，该系统具有用于制备组织（Magna rymerer），核酸（Magna Pure Compact）和实时PCR（LightCyccler 480）的自动组件（Magna pure Compact）的分离。 LightCycler 480是一种高吞吐量实时PCR仪器，可检测和量化核酸序列，并具有96孔和384孔板的容量。这种PCR工作流程系统非常易于使用，伴随着功能强大的软件，并使研究人员能够在3-4小时内从组织到核酸定量。该乐器将安置在大学的Bosomworth Health Sciences Research大楼的微阵列核心研究机构中，并将在毒理学研究生中心以及解剖学和神经生物学系中使用。参加此申请的调查人员小组包括七个拥有NIH支持的赠款项目的教职员工，他们是主要用户，另外三名是未成年人的教职员工。研究项目是多种多样的，但围绕三个主要主题旋转：1）氧化剂应激的组织损伤和癌症，2）DNA损伤，修复和诱变以及3）基因表达的激素调节。我们打算使用该仪器对生物样品中的RNA转录本和DNA靶标进行高通量实时定量分析，并检测到重要的癌症基因中的单核苷酸多态性（SNP）。正如每个研究人员的研究项目所示，所提出的工具的可用性将显着提高其研究的范围和及时性。最重要的是，这将使科学能够在其资助的研究项目中确定对令人兴奋的发现的追求，而不是当前工具的可用性。这种共享的多学科研究机构的总体目标是利用这种先进的技术资源来进行当今生物医学研究的尖端研究，并培训我们的博士生和博士后研究员，以使用这些现代方法，以使他们能够发展为成功的独立研究人员。我们的研究人员解决的研究问题与与癌症有关的主要公共卫生问题高度相关，包括肿瘤形成机制，（缺乏）癌症化学治疗药物有效性的原因以及对女性健康重要的激素调节基因。