Integrated instrument system for maintenance and delivery of RNAi libraries

用于维护和交付 RNAi 文库的集成仪器系统

• 批准号: 7388756
• 负责人: Mariano A. Garcia-Blanco
• 金额: $ 27.66万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-02-15 至 2009-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388756
• 关键词: Affect; Arts; Automation; Biology; Cell physiology; Cells; Communities; Computers; Coupled; Development; Disease; Duke Comprehensive Cancer Center; Genes; Genetic; Genome; Image; In Vitro; Institutes; Investments; Knowledge; Libraries; Lipids; Liquid substance; Maintenance; Mammalian Cell; Mission; Molecular; Pathway interactions; Pliability; RNA; RNA Interference; Reagent; Research; Research Infrastructure; Research Personnel; Resource Sharing; Role; Science Policy; Screening procedure; Small Interfering RNA; System; Technology; Transfection; Viral; base; design; experimental analysis; functional genomics; gene function; improved; in vivo; instrument; loss of function; programs; quantum; seal; small molecule libraries; tool

DESCRIPTION (provided by applicant): The advent of RNA interference as a mechanism for post-transcriptional silencing of gene function in mammalian cells represents a quantum advance and tremendous opportunity for experimental analysis of gene function. With the development of genome-wide libraries of siRNAs and shRNAs, we have entered a new era in which the application of powerful forward genetic approaches can be used both in vivo and in vitro to assign function to genes, delineate molecular pathways in which these genes affect normal and disease cellular processes, and contribute to the knowledge necessary to develop new and improve existing therapies. The Duke Center for RNA Biology, the Duke Comprehensive Cancer Center, and the Duke Institute for Genome Sciences and Policy, have come together to establish an RNAi shared-resource facility. A primary mission of the RNAi Facility is to develop and deploy RNAi technologies to support functional genomics research programs at Duke. Through large investments in RNAi and complementary technologies, we are assembling a state-of-the-art infrastructure for functional genomics, providing researchers access to genome- wide RNAi reagents and the infrastructure necessary to conduct large-scale loss-of-function studies. In this proposal, we seek support to significantly enhance this technology infrastructure with the acquisition of the Velocity 11 Integrated Instrument System for Maintenance and Delivery of RNAi/Small Molecule Libraries. This instrument system will provide the automation required for RNAi library formatting, plate handling, liquid dispensing, plate sealing and plate identification/management. This instrument will complete the suite of tools necessary to make whole genome RNAi based screening a reality for the Duke research community. The Velocity 11 system was designed from the ground up as a fully integrated computer controlled instrument and is pre-configured by Velocity 11 for the multiple workflow options necessary to maintain and deliver siRNA libraries via lipid based transfection or viral based transduction of mammalian cells while maintaining the flexibility to be useful in small molecule library screening. Coupled with our existing downstream High-content cell based imaging system, whole genome RNAi based screening represents a tremendous technological advance, enabling researchers to identify genes with roles in virtually any normal or disease-associated cellular process.

描述（由申请人提供）：RNA干扰作为哺乳动物细胞中基因功能的转录后沉默的一种机制的出现，代表了对基因功能实验分析的量子进步和巨大的机会。随着siRNA和shRNA的全基因组库的发展，我们进入了一个新时代，在该时代中，可以在体内和体外使用强大的远期遗传方法的应用来将功能分配给基因，从而描述这些基因的分子途径，其中这些基因影响正常和疾病的细胞过程，并为知识带来必要的知识，从而发展新的和改善现有的疗法。杜克大学RNA生物学中心，杜克大学综合癌症中心和杜克大学基因组科学与政策研究所，已经建立了RNAi共享资源设施。 RNAi设施的主要任务是开发和部署RNAi技术，以支持杜克大学的功能基因组学研究计划。通过对RNAi和互补技术的大量投资，我们正在为功能基因组学提供最新的基础设施，从而为研究人员提供了对基因组广泛的RNAi试剂的访问，以及进行大规模丧失函数丧失研究所需的基础设施。在此提案中，我们寻求支持，通过获取速度11集成仪器系统来维护和交付RNAi/小分子库，从而显着增强了该技术基础架构。该仪器系统将提供RNAi库格式，板处理，液体分配，板密封和板识别/管理所需的自动化。该工具将完成必要的工具套件，以使整个基因组基于RNAi的筛查成为杜克大学研究社区的现实。速度11系统是从头开始设计的，作为一种完全集成的计算机控制仪器，并由速度11预先配置，用于通过基于脂质的转染或基于病毒的基于病毒的哺乳动物细胞的转染，同时维持smamalian细胞，同时保持灵活性在小分子库中有用。再加上我们现有的下游高素质细胞成像系统，整个基因组基于RNAi的筛选代表了巨大的技术进步，使研究人员能够鉴定出在几乎任何正常或疾病相关的细胞过程中具有作用的基因。