Confocal Microscope

共焦显微镜

• 批准号: 7383535
• 负责人: Mark R Ackermann
• 金额: $ 43.39万
• 依托单位: IOWA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-04-15 至 2010-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7383535
• 关键词: Age; Annual Reports; Arts; Biotechnology; Cells; Chromatin Structure; Confocal Microscopy; Fascicle; Fluorescence; Fluorescence Resonance Energy Transfer; Funding; Gene Expression; Gene Targeting; Housing; Image Analysis; Iowa; Life; Light; Maintenance; Microscope; Minor; Neurons; Oxidative Stress; Phosphotransferases; Public Health; Range; Rate; Research Personnel; Resolution; Retrotransposon; Scanning; Signal Transduction; Stem Cell Development; Stifle joint; Surveys; System; Training; United States National Institutes of Health; Universities; aptamer; cellular imaging; experience; instrumentation; radius bone structure; reconstruction

DESCRIPTION (provided by applicant): State-of-the art confocal microscopy is required for NIH-funded projects at Iowa State University (ISU). The existing multi-user confocal system at ISU is housed and operated by the Image Analysis and Confocal Microscopy Facility, but a new system is needed, because: 1) the existing Leica System TCS/NT (1998) is aging, cannot handle the live cell imaging needs of major users, and lacks needed accessories such as FRET and 3D- reconstruction, and 2) no other confocal system with these capabilities is available to ISU investigators for a 100 mile radius. Through survey and discussion, we have determined that a Leica TCS SP5 would provide the fast scan rates, high resolution, high sensitivity to reduce light load and damage to cells, and ability to fine tune excitation and emission channels to aid in simultaneous acquisition of multiple fluorescence channels needed for five major NIH-funded projects as well as minor projects, some also NIH-funded, outlined in this proposal. These investigators have much experience in confocal microscopy, but are stifled by the limitations of our existing system. Major user projects include: axonal fascicle formation, JIL-1 kinase effects on chromatin structure and gene expression, PKC4 signaling, oxidative stress, and dopaminergic degeneration in neurons, aptamer tags for assessing gene expression and neuronal stem cell development and plasticity, and retrotransposon gene targeting. The Image Analysis and Confocal Facility at ISU is also well suited to handle this new system for the major and minor users. The facility is supported by the Office of Biotechnology at ISU, has existed since 1984. Ackermann (PI) with Carter (collaborator) acquired, operated, and managed the existing confocal system since 1998. Ackermann is a minor user in this proposal, but has an R01- funded project. The facility handles all aspects of the confocal system (maintenance, training, usage, upgrades, etc.) and has had an active user committee since inception and provides annual reports to the Council of the Office of Biotechnology. This instrumentation is relevant to public health in terms of supporting already funded NIH- projects that range from a better understanding of gene expression to neuronal stem cell development and plasticity.

描述（由申请人提供）：爱荷华州立大学（ISU）的NIH资助项目需要最先进的共聚焦显微镜。 ISU现有的多用户共同系统是由图像分析和共同体显微镜设施容纳和操作的，但是需要一个新系统，因为：1）现有的Leica System TCS/NT（1998）正在衰老，无法处理主要用户的实时成像需求，以及与Freet和3D-IS构造的100次启动者，以及这些不可用的IS构造和2），并且这些insuboluction and Is构造和2）不适合这些兴趣。半径。通过调查和讨论，我们已经确定Leica TCS SP5将提供快速扫描速率，高分辨率，高敏感性，以减少光负载和对细胞的损害，以及对五个大型NIH资助的项目所需的多个荧光渠道进行微调和发射渠道的能力，并有助于同时获得多个荧光频道，以及一些较小的项目，以及一些不受限制地付诸实践的，这些项目也无效。这些研究人员在共聚焦显微镜上具有很大的经验，但被我们现有系统的局限性所扼杀。主要用户项目包括：轴突筋膜形成，JIL-1激酶对染色质结构和基因表达的影响，神经元中神经元的PKC4信号传导，氧化应激和多巴胺能变性，适体标签，用于评估基因表达和神经元干细胞发育和可塑性以及转化转移基因的基因表达和基因基因靶标。 ISU的图像分析和共聚焦设施也非常适合为主要用户和次要用户处理这一新系统。该设施得到了ISU的生物技术办公室的支持，自1984年以来就一直存在。自1998年以来，Ackermann（PI）与Carter（Collaborator）自1998年以来收购，操作和管理现有共聚焦系统。Ackermann是此提案中的次要用户，但具有R01资助的项目。该设施处理共聚焦系统的各个方面（维护，培训，使用，升级等），并且自成立以来就已经有一个活跃的用户委员会，并向生物技术办公室提供了年度报告。这种仪器与公共卫生有关，就支持已经资助的NIH-项目从更好地理解基因表达到神经元干细胞的发育和可塑性。