Mechanisms of Articular Cartilage Lubrication

关节软骨润滑的机制

• 批准号: 7417751
• 负责人: Robert L Sah
• 金额: $ 32.34万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-10 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7417751
• 关键词: Address; Adherence; Adsorption; Affect; Aging; Arthritis; Biomechanics; Biomedical Engineering; Cartilage; Cartilage Matrix; Cell Line; Chondrocytes; Condition; Degenerative polyarthritis; Depth; Deterioration; Diagnostic; Disease; Equus caballus; Exhibits; Failure; Friction; Health; Hyaluronan; In Vitro; Injury; Joints; Liquid substance; Lubricants; Lubrication; Maintenance; Mechanics; Membrane Proteins; Modeling; Molecular; Numbers; Osteoarthrosis Deformans; Phospholipids; Physiological; Positioning Attribute; Process; Property; Proteins; Protocols documentation; Public Health; Regulation; Relative (related person); Research Personnel; Slide; Solutions; Stimulus; Surface; Synovial Fluid; System; Testing; Therapeutic; Tissues; Torsion; Weight-Bearing state; articular cartilage; base; cytokine; injured; insight; interest; lubricin; osteochondral tissue; programs; protein metabolism; research study

Articular cartilage is one of a number of tissues in the body that bears load and slides relative to an apposing tissue surface with remarkable tribological properties¿low friction, low wear, and multiple modes of lubrication acting to lower friction and/or wear. However, in aging and osteoarthritis, the articular surface becomes roughened and eroded. Such deterioration may be the result of several factors leading to excessive loading, a failure in lubrication mechanisms, or both. The broad, long-term objectives are to contribute to the understanding of the mechanobiological system of the interacting joint compartments in order to gain insight into the mechanisms and consequences of lubrication of articular cartilage in joints. This proposal seeks to advance the understanding of lubrication mechanisms, in particular the molecular basis for boundary-mode lubrication of articular cartilage by synovial fluid. The overall hypothesis to be tested is that boundary lubrication of articular cartilage occurs via interactions of synovial fluid molecules with the articular surface, reducing boundary-mode friction and wear, and thereby contributing to the mechanical maintenance of healthyjoints. This hypothesis suggests that the concentration of certain molecules within the joint cavity and their adherence at the articular surface are critical determinants of functional lubrication. The specific hypotheses to be tested are: Hypothesis 1: The SF components that lower friction at a cartilage-cartilage interface via boundary lubrication are Lub/SZP, SAPL, and HA, acting alone or in combination. Hypothesis 2: The SF components that lower wear at a cartilage-cartilage interface via boundary lubrication are Lub/SZP, SAPL, and HA, acting alone or in combination. Hypothesis 3: Solutions of biosynthetic and actual SF provide articular cartilage with friction and wear properties according to levels of Lub/SZP, SAPL, and HA, and results of the studies addressing Hypotheses 1 and 2. To address each hypothesis, studies are proposed beginning with tissue-level studies on excised osteochondral fragments in a cartilage-on-cartilage test configuration. They proceed to selected joint-scale biomechanical experiments on articulating cartilage. Relevance to Public Health: Confirmation of the above hypotheses is relevant both to the normal maintenance of joint health and to pathogenic processes in arthritic disease. Such information may ultimately facilitate diagnostic analysis and therapeutic manipulation of molecules of mechanical importance in the synovial fluid compartment.

人工软骨是体内许多组织之一，相对于一个载荷和滑动 具有显着摩擦学特性的组织表面效果低摩擦，低磨损和多种模式的 润滑作用于降低摩擦和/或磨损。但是，在衰老和骨关节炎中，关节表面 变得粗糙和侵蚀。这种定义可能是导致过量过量因素的几个因素的结果 负载，润滑机制的故障或两者兼而有之。广泛的长期目标是为 了解相互作用的关节隔室的机械系统，以获得洞察力 进入关节中关节软骨润滑的机理和后果。该提议试图 提高对润滑机制的理解，尤其是边界模式的分子基础 通过滑液对关节软骨的润滑。 要测试的总体假设是关节软骨的边界润滑是通过相互作用发生的 带有关节表面的滑液分子，减少边界模式摩擦和磨损，从而减少 有助于机械维护健康偶像。该假设表明浓度 关节腔内的某些分子及其在关节表面的粘附是关键的决定剂 功能润滑。要测试的具体假设是： 假设1：通过边界润滑在软骨 - 核心界面处降低摩擦的SF成分 是单独或组合起作用的lub/szp，sapl和ha。 假设2：通过边界润滑在软骨 - 卡托尔界面下降低磨损的SF组件 是单独或组合起作用的lub/szp，sapl和ha。 假设3：生物合成和实际SF的溶液提供摩擦和磨损的关节软骨 根据LUB/SZP，SAPL和HA的水平的性质，以及针对解决的结果 假设1和2。 为了解决每个假设，提出的研究是从组织级研究开始的 软骨片剂测试配置中的骨软骨片段。他们继续选择联合尺度 关于阐释软骨的生物力学实验。 与公共卫生的相关性：上述假设的确认都与正常 维持联合健康和artritic疾病的致病过程。这样的信息可能最终 促进对机械重要性分子的诊断分析和治疗性操纵 滑液室。